PMID- 7659527
OWN - NLM
STAT- MEDLINE
DCOM- 19951002
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 23
IP  - 15
DP  - 1995 Aug 11
TI  - Increasing binding of a transcription factor immediately downstream of the cap 
      site of a cytomegalovirus gene represses expression.
PG  - 3026-33
AB  - A closely related family of ubiquitous DNA binding proteins, called MDBP, binds 
      with high affinity to two 14 base pair (bp) sites within the human 
      cytomegalovirus immediate early gene 1 (CMV IE1) enhancer and with low affinity 
      to one site beginning 5 bp downstream of the CMV IE1 transcription start point 
      (+5 site). Unlike several cap position downstream MDBP sites in mammalian genes, 
      these MDBP sites do not require cytosine methylation for optimal binding. 
      Mutation of one of the enhancer MDBP sites to prevent MDBP recognition modestly 
      increased the function of a neighboring CREB binding site in a transient 
      transfection assay in the context of one promoter construct. A much larger effect 
      on reporter gene expression (a 10-fold reduction) was seen when the low affinity 
      MDBP recognition sequence at position +5 was converted to a high affinity site in 
      a plasmid containing the CMV IE1 promoter upstream of the reporter gene. Evidence 
      that the increased binding of MDBP at the mutant site is largely responsible for 
      the observed results was provided by transfection experiments with this high 
      affinity MDBP +5 site re-mutated to a non-binding site and by in vitro 
      transcription assay.
FAU - Zhang, X Y
AU  - Zhang XY
AD  - Department of Biochemistry, Tulane Medical School, New Orleans, LA 70112, USA.
FAU - Ni, Y S
AU  - Ni YS
FAU - Saifudeen, Z
AU  - Saifudeen Z
FAU - Asiedu, C K
AU  - Asiedu CK
FAU - Supakar, P C
AU  - Supakar PC
FAU - Ehrlich, M
AU  - Ehrlich M
LA  - eng
GR  - GM33999/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MBD1 protein, human)
RN  - 0 (Polydeoxyribonucleotides)
RN  - 0 (Transcription Factors)
SB  - IM
GS  - IE1
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cytomegalovirus/*genetics
MH  - DNA, Viral/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Enhancer Elements, Genetic/genetics
MH  - Gene Expression Regulation, Viral/*genetics
MH  - Genes, Immediate-Early/genetics
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Polydeoxyribonucleotides/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC307145
EDAT- 1995/08/11 00:00
MHDA- 1995/08/11 00:01
CRDT- 1995/08/11 00:00
PHST- 1995/08/11 00:00 [pubmed]
PHST- 1995/08/11 00:01 [medline]
PHST- 1995/08/11 00:00 [entrez]
AID - 5a0111 [pii]
AID - 10.1093/nar/23.15.3026 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1995 Aug 11;23(15):3026-33. doi: 10.1093/nar/23.15.3026.