PMID- 7659683
OWN - NLM
STAT- MEDLINE
DCOM- 19951002
LR  - 20191023
IS  - 0893-5785 (Print)
IS  - 0893-5785 (Linking)
VI  - 8
IP  - 2
DP  - 1995 Apr
TI  - The expression of tyrosinase, tyrosinase-related proteins 1 and 2 (TRP1 and 
      TRP2), the silver protein, and a melanogenic inhibitor in human melanoma cells of 
      differing melanogenic activities.
PG  - 97-104
AB  - The expression of various melanogenic proteins, including tyrosinase, the 
      tyrosinase-related proteins 1 (TRP1) and 2 (TRP2/DOPAchrome tautomerase), and the 
      silver protein in human melanocytes was studied in six different human melanoma 
      cell lines and compared to a mouse derived melanoma cell line. Analysis of the 
      expression of tyrosinase, TRP1, TRP2, and the silver protein using flow cytometry 
      revealed that in general there was a positive correlation between melanin 
      formation and the expression of those melanogenic enzymes. Although several of 
      the melanoma cell lines possessed significant activities of TRP2, the levels of 
      DOPAchrome tautomerase in extracts of human cells were relatively low compared to 
      those in murine melanocytes. Melanins derived from melanotic murine JB/MS cells, 
      from melanotic human Ihara cells and HM-IY cells, from sepia melanin, and from 
      C57BL/6 mouse hair were chemically analyzed. JB/MS cells, as well as Ihara cells 
      and HM-TY cells, possessed significant amounts of 
      5,6-dihydroxyindole-2-carboxylic acid (DHICA) derived melanins, this being 
      dependent on the activity of TRP2. Kinetic HPLC assays showed that 
      5,6-dihydroxyindole (DHI) produced during melanogenesis was metabolized quickly 
      to melanin in pigmented KHm-1/4 cells, whereas DHI was stable in amelanotic human 
      SK-MEL-24 cells. A melanogenic inhibitor that has been purified from SK-MEL-24 
      cells that suppressed oxidation of DHI in the presence or absence of tyrosinase, 
      but had no effect on DHICA oxidation. The sum of these results suggests that the 
      expression of melanogenic enzymes as well as the activity of a melanogenic 
      inhibitor are critical to the production of melanin synthesis in humans.
FAU - Kameyama, K
AU  - Kameyama K
AD  - Department of Dermatology, Kitasato Institute Medical Center Hospital, Saitama, 
      Japan.
FAU - Sakai, C
AU  - Sakai C
FAU - Kuge, S
AU  - Kuge S
FAU - Nishiyama, S
AU  - Nishiyama S
FAU - Tomita, Y
AU  - Tomita Y
FAU - Ito, S
AU  - Ito S
FAU - Wakamatsu, K
AU  - Wakamatsu K
FAU - Hearing, V J
AU  - Hearing VJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Pigment Cell Res
JT  - Pigment cell research
JID - 8800247
RN  - 0 (Melanins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteins)
RN  - 0 (Silver Proteins)
RN  - 9002-79-3 (Melanocyte-Stimulating Hormones)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (TYRP1 protein, human)
RN  - EC 1.14.18.- (Tyrp1 protein, mouse)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - EC 5.- (Isomerases)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Flow Cytometry
MH  - Humans
MH  - *Intramolecular Oxidoreductases
MH  - Isomerases/*metabolism
MH  - Melanins/*antagonists & inhibitors/*biosynthesis
MH  - Melanocyte-Stimulating Hormones/pharmacology
MH  - Melanoma/*metabolism
MH  - *Membrane Glycoproteins
MH  - Mice
MH  - Monophenol Monooxygenase/*metabolism
MH  - *Oxidoreductases
MH  - Proteins/*metabolism
MH  - Silver Proteins/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1111/j.1600-0749.1995.tb00648.x [doi]
PST - ppublish
SO  - Pigment Cell Res. 1995 Apr;8(2):97-104. doi: 10.1111/j.1600-0749.1995.tb00648.x.