PMID- 7661693
OWN - NLM
STAT- MEDLINE
DCOM- 19951003
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 140
IP  - 8
DP  - 1995
TI  - Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory 
      syndrome virus, Quebec reference strain.
PG  - 1405-18
AB  - The cDNA sequence of the 3'-terminal genomic region of the Quebec IAF-exp91 
      strain of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      determined and compared to those of other reference strains from Europe (Lelystad 
      virus) and US (ATCC VR2385, MN-1b). The sequence (2834 nucleotides) which 
      encompassed ORFs 3 to 7 revealed extensive genomic variations between the Quebec 
      strain and Lelystad virus (LV), resulting from high number of base substitutions, 
      additions and deletions. The ORFs 5, 3, and 7 seemed to be relatively the most 
      variable; the predicted encoding products of the Quebec and LV strains displayed 
      only 52%, 54%, and 59% amino acid identities, respectively. Nevertheless, in 
      vitro translation experiments of the structural genes (ORFs 5, 6, and 7) and 
      radioimmunoprecipitation assays with extracellular virions gave results similar 
      to those previously reported for LV. In contrast, close genomic relationships 
      were demonstrated between Quebec and US strains. Taking together, these results 
      indicate that, although structurally similar, North American PRRSV strains belong 
      to a genotype distinct from that of the LV, thus supporting previous findings 
      that allowed to divide PRRSV isolates into two antigenic subgroups (U.S. and 
      European).
FAU - Mardassi, H
AU  - Mardassi H
AD  - Centre de recherche en virologie, Institut Armand-Frappier, Universite du Quebec, 
      Laval, Canada.
FAU - Mounir, S
AU  - Mounir S
FAU - Dea, S
AU  - Dea S
LA  - eng
SI  - GENBANK/L40898
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Complementary)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arterivirus/chemistry/classification/*genetics
MH  - Base Sequence
MH  - Conserved Sequence
MH  - DNA, Complementary/genetics
MH  - Europe
MH  - *Genes, Viral
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Quebec
MH  - Swine/virology
MH  - Viral Structural Proteins/*genetics
PMC - PMC7087035
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 2020/03/23
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - BF01322667 [pii]
AID - 10.1007/BF01322667 [doi]
PST - ppublish
SO  - Arch Virol. 1995;140(8):1405-18. doi: 10.1007/BF01322667.