PMID- 7661877 OWN - NLM STAT- MEDLINE DCOM- 19951005 LR - 20190718 IS - 0021-9150 (Print) IS - 0021-9150 (Linking) VI - 115 IP - 2 DP - 1995 Jun TI - Cysteinyl-leukotriene D4 induced IL-1 beta expression and release in rat vascular smooth muscle cells. PG - 181-9 AB - Vascular cells, including smooth muscle cells (VSMC), may release interleukin 1 (IL-1) and transcribe its genes for both isoforms. Previous studies have shown that cysteinyl-leukotrienes can modulate cytokine production by monocytes and a cytokine-eicosanoid network has been suggested during atherosclerosis. In this study the effects of cysteinyl-leukotriene D4 (LTD4) on IL-1 beta production and IL-1 beta mRNA expression were tested on rat VSMC. LTD4 showed a significant dose-dependent (from basal production of 55 +/- 15 pg/ml to maximal production of 177 +/- 14 pg/ml) and time-dependent (peaking at 24 h 16 +/- 54 pg/ml) increase of IL-1 beta immunoreactivity in the supernatants of conditioned medium and cell lysates. Furthermore, LTD4 induced an increased mRNA expression which began at 1 h and peaked at 12 h incubation time. The production of IL-1 beta was inhibited by MK-571 (from 145 +/- 12 to 60 +/- 10 pg/ml), a specific receptor antagonist of LTD4 and partially reduced by IL-1 receptor antagonist (IL-1 ra) (from 160 +/- 12 to 85 +/- 5 pg/ml). These experiments suggest that cysteinyl-leukotrienes, potentially produced in the vascular wall by leukocytes or by transcellular metabolism, may be involved in local IL-1 production. FAU - Porreca, E AU - Porreca E AD - Istituto di Patologia Speciale Medica, Ospedale Civile SS Annunziata, Chieti, Italy. FAU - Conti, P AU - Conti P FAU - Feliciani, C AU - Feliciani C FAU - Di Febbo, C AU - Di Febbo C FAU - Reale, M AU - Reale M FAU - Mincione, G AU - Mincione G FAU - Neri, M AU - Neri M FAU - Amerio, P AU - Amerio P FAU - Cuccurullo, F AU - Cuccurullo F LA - eng PT - Journal Article PL - Ireland TA - Atherosclerosis JT - Atherosclerosis JID - 0242543 RN - 0 (Interleukin-1) RN - 0 (Propionates) RN - 0 (Quinolines) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Interleukin-1) RN - 5Q9O54P0H7 (verlukast) RN - 73836-78-9 (Leukotriene D4) SB - IM MH - Analysis of Variance MH - Animals MH - Aorta/cytology/drug effects/metabolism MH - Cells, Cultured MH - Dose-Response Relationship, Drug MH - Gene Expression Regulation/drug effects MH - Interleukin-1/*biosynthesis/genetics/metabolism MH - Leukotriene D4/*pharmacology MH - Muscle, Smooth, Vascular/cytology/*drug effects/metabolism MH - Propionates/pharmacology MH - Quinolines/pharmacology MH - RNA, Messenger/drug effects/metabolism MH - Rats MH - Receptors, Interleukin-1/antagonists & inhibitors EDAT- 1995/06/01 00:00 MHDA- 1995/06/01 00:01 CRDT- 1995/06/01 00:00 PHST- 1995/06/01 00:00 [pubmed] PHST- 1995/06/01 00:01 [medline] PHST- 1995/06/01 00:00 [entrez] AID - 0021-9150(94)05510-P [pii] AID - 10.1016/0021-9150(94)05510-p [doi] PST - ppublish SO - Atherosclerosis. 1995 Jun;115(2):181-9. doi: 10.1016/0021-9150(94)05510-p.