PMID- 7662193
OWN - NLM
STAT- MEDLINE
DCOM- 19951006
LR  - 20190718
IS  - 0269-9370 (Print)
IS  - 0269-9370 (Linking)
VI  - 9
IP  - 6
DP  - 1995 Jun
TI  - Effects of primary HIV-1 infection on subsets of CD4+ and CD8+ T lymphocytes.
PG  - 561-6
AB  - OBJECTIVE: To analyse changes in T-lymphocyte subsets in patients with primary 
      HIV infection and to determine their specificity (and therefore their diagnostic 
      utility) by comparing these changes with those seen in other acute illnesses as 
      well as in HIV-uninfected patients. METHODS: T-lymphocyte subsets were analysed 
      by two- and three-colour flow cytometry, and compared between HIV seroconverters 
      (n = 16), HIV-infected (n = 18) and uninfected (n = 33) controls, patients with 
      infectious mononucleosis (n = 7), and patients suspected clinically of having 
      primary HIV infection but who were later found to be uninfected (referred to as 
      HIV non-converters; n = 17). RESULTS: CD4+ lymphocyte counts were significantly 
      lower in HIV seroconverters (mean, 444 x 10(6)/l) than in the HIV non-converters 
      (793 x 10(6)/l; P = 0.003), HIV-seronegative controls (888 x 10(6)/l; P < 0.0001) 
      and, to a lesser extent, those with mononucleosis (694 x 10(6)/l; P = 0.045). The 
      reduction in CD4+ lymphocytes occurred in both the CD45RA+ (55%) and CD45R0+ 
      (33%) subsets. CD8+ lymphocyte counts were significantly higher in HIV 
      seroconverters (942 x 10(6)/l) than in HIV non-converters (570 x 10(6)/l; P = 
      0.003) and seronegative controls (467 x 10(6)/l; P < 0.0001), but significantly 
      lower than in the mononucleosis group (3682 x 10(6)/l; P = 0.004). The CD8+ cells 
      in the HIV seroconverters had increased coexpression of CD45R0, human leukocyte 
      antigen (HLA)-DR, CD38 and CD11a/CD18. The mean CD4: CD8 ratio in the HIV 
      seroconverters was 0.49, versus 1.52 in the non-converters (P < 0.0001), 2.08 in 
      the seronegative patients (P < 0.0001) and 0.37 in the mononucleosis patients (P 
      > 0.2). CONCLUSIONS: Primary HIV infection is characterized by a depletion of 
      CD4+ lymphocytes, especially of the CD45RA+ phenotype, and by an increase in CD8+ 
      lymphocytes with an activated phenotype; the latter was also seen in patients 
      with infectious mononucleosis but not in HIV non-converters or HIV-seronegative 
      patients. Patients suspected clinically of having primary HIV infection but with 
      normal T-cell phenotype are less likely to have primary HIV infection. These 
      phenotypic changes, as well as an inverted CD4: CD8 ratio, can readily 
      distinguish patients with primary HIV infection from HIV-uninfected patients 
      except those with infectious mononucleosis. Therefore, T-cell-subset enumeration 
      may be useful in the diagnosis of primary HIV infection.
FAU - Zaunders, J
AU  - Zaunders J
AD  - Centre for Immunology, St Vincent's Hospital, Sydney, New South Wales, Australia.
FAU - Carr, A
AU  - Carr A
FAU - McNally, L
AU  - McNally L
FAU - Penny, R
AU  - Penny R
FAU - Cooper, D A
AU  - Cooper DA
LA  - eng
PT  - Journal Article
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*immunology/pathology
MH  - Adult
MH  - Antigens, CD/analysis/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/pathology
MH  - CD8-Positive T-Lymphocytes/*immunology/pathology
MH  - Cell Count
MH  - Female
MH  - Flow Cytometry
MH  - HIV-1/*immunology
MH  - HLA-DR Antigens/analysis/immunology
MH  - Humans
MH  - Male
MH  - T-Lymphocyte Subsets
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1097/00002030-199506000-00005 [doi]
PST - ppublish
SO  - AIDS. 1995 Jun;9(6):561-6. doi: 10.1097/00002030-199506000-00005.