PMID- 7664245
OWN - NLM
STAT- MEDLINE
DCOM- 19951011
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 82
IP  - 2
DP  - 1995 Jul 15
TI  - Identification of two distinct chromosome 12-derived amplification units in 
      neuroblastoma cell line NGP.
PG  - 151-4
AB  - The neuroblastoma cell line NGP contains two homogeneously staining regions 
      (hsr). One of these hsrs contains MYCN sequences. Reverse painting experiments 
      demonstrated that the second HSR consisted of two chromosome 12-derived 
      amplification units, located at 12q14-15 and 12q24. Southern blot and 
      fluorescence in situ hybridization (FISH) analysis showed amplification of genes 
      located at 12q14-15: SAS, MDM2, and CDK4, GLI, CHOP, CDK2, and A2MR were found 
      not to be amplified. FISH further demonstrated amplification of RSN, a gene 
      located at 12q24. The finding of two distinct chromosome 12 amplification units 
      in a neuroblastoma cell line NGP is reminiscent of recent findings in 
      well-differentiated liposarcoma (WDLPS) and other sarcomas. The second 
      amplification unit on chromosome 12 in NGP is located more distal (12q24) than 
      the one observed in WDLPS (12q21). The mechanism and biologic significance of 
      this amplification process in neuroblastoma and WDLPS remain to be elucidated.
FAU - Van Roy, N
AU  - Van Roy N
AD  - Department of Medical Genetics, University Hospital, Gent, Belgium.
FAU - Forus, A
AU  - Forus A
FAU - Myklebost, O
AU  - Myklebost O
FAU - Cheng, N C
AU  - Cheng NC
FAU - Versteeg, R
AU  - Versteeg R
FAU - Speleman, F
AU  - Speleman F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosomes, Human, Pair 12
MH  - DNA, Neoplasm/analysis
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neuroblastoma/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1995/07/15 00:00
MHDA- 1995/07/15 00:01
CRDT- 1995/07/15 00:00
PHST- 1995/07/15 00:00 [pubmed]
PHST- 1995/07/15 00:01 [medline]
PHST- 1995/07/15 00:00 [entrez]
AID - 016546089500034M [pii]
AID - 10.1016/0165-4608(95)00034-m [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Jul 15;82(2):151-4. doi: 
      10.1016/0165-4608(95)00034-m.