PMID- 7664729
OWN - NLM
STAT- MEDLINE
DCOM- 19951010
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 14
IP  - 16
DP  - 1995 Aug 15
TI  - Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: 
      expression during sporozoite ontogeny and binding to human hepatocytes.
PG  - 3883-94
AB  - Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of 
      the mosquito show profound differences in their biological properties such as 
      motility, ability to induce protective immune response and infectivity for 
      vertebrate host cells. Sporozoites from salivary glands are much more infectious 
      than those from oocysts and haemocoel. Differential expression of proteins, such 
      as the circumsporozoite (CS) protein and the thrombospondin-related adhesive 
      protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes 
      may account for the development of infectivity during ontogeny. We have carried 
      out a series of experiments to: (i) analyse the expression and localization of 
      TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) 
      elucidate the biochemical and adhesive properties of recombinant TRAP. Our data 
      indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS 
      protein are found in this parasite developmental stage. Hemocoel sporozoites 
      display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a 
      frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ 
      CS protein+. We also provide experimental evidence showing that recombinant TRAP 
      binds to the basolateral cell membrane of hepatocytes in the Disse's space and 
      that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.
FAU - Robson, K J
AU  - Robson KJ
AD  - MRC Molecular Haematology Unit, John Radcliffe Hospital, Headington, Oxford, UK.
FAU - Frevert, U
AU  - Frevert U
FAU - Reckmann, I
AU  - Reckmann I
FAU - Cowan, G
AU  - Cowan G
FAU - Beier, J
AU  - Beier J
FAU - Scragg, I G
AU  - Scragg IG
FAU - Takehara, K
AU  - Takehara K
FAU - Bishop, D H
AU  - Bishop DH
FAU - Pradel, G
AU  - Pradel G
FAU - Sinden, R
AU  - Sinden R
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glycoconjugates)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sulfuric Acids)
RN  - 0 (circumsporozoite protein, Protozoan)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
RN  - O40UQP6WCF (sulfuric acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anopheles/parasitology
MH  - Antibodies, Monoclonal
MH  - Base Sequence
MH  - Glycoconjugates/metabolism
MH  - Humans
MH  - Liver/cytology/*metabolism/parasitology
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Plasmodium falciparum/*growth & development/metabolism
MH  - Protozoan Proteins/*biosynthesis/chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/isolation & purification/metabolism
MH  - Salivary Glands/parasitology
MH  - Sulfuric Acids
MH  - Tumor Cells, Cultured
PMC - PMC394467
EDAT- 1995/08/15 00:00
MHDA- 1995/08/15 00:01
PMCR- 1996/08/15
CRDT- 1995/08/15 00:00
PHST- 1995/08/15 00:00 [pubmed]
PHST- 1995/08/15 00:01 [medline]
PHST- 1995/08/15 00:00 [entrez]
PHST- 1996/08/15 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1995.tb00060.x [doi]
PST - ppublish
SO  - EMBO J. 1995 Aug 15;14(16):3883-94. doi: 10.1002/j.1460-2075.1995.tb00060.x.