PMID- 7665184
OWN - NLM
STAT- MEDLINE
DCOM- 19951012
LR  - 20190904
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 27
IP  - 1
DP  - 1995 May 1
TI  - A continuous high-resolution physical map spanning 17 megabases of the q12, 
      q13.1, and q13.2 cytogenetic bands of human chromosome 19.
PG  - 52-66
AB  - We report the construction of a high-resolution physical map of a 17-Mb region 
      that encompasses the entire q12, q13.1, and q13.2 bands of human chromosome 19. 
      The continuous map extends from a region approximately 400 kb centromeric of the 
      D19S7 marker to the excision repair cross-complementing rodent repair deficiency 
      complementation group 1 (ERCC1) locus. The ordered clone map has been obtained 
      starting from a foundation of cosmid contigs assembled by automated 
      fingerprinting and localized to the cytogenetic map by fluorescence in situ 
      hybridization (FISH). Clonal continuity of the map has been achieved by binning 
      and linking the premapped cosmid contigs by means of yeast artificial chromosomes 
      (YACs). The map consists of a single contig composed of 169 YAC members (minimal 
      spanning path of 18 YACs) linking 165 cosmid contigs. Eighty percent, or about 
      13.2 Mb of the entire region spanned by the map, has been resolved to the EcoRI 
      restriction map level. Twenty-nine sequence-tagged sites associated with genetic 
      markers or derived from FISH-mapped cosmids have been placed on the map. In 
      addition to the ERCC1 gene area, the map includes the location of the creatine 
      kinase muscle locus (CKM), imidazoledipetidase (PEPD), glucophosphate isomerase 
      (GPI), myelin-associated glycoprotein (MAG), the apolipoprotein E and C (APOE and 
      APOC) genes, and the ryanodine receptor (RYR1) gene. This type of map provides a 
      source of continuously overlapping DNA segments at a level of resolution two 
      orders of magnitude higher than that obtained using YACs alone. In addition, it 
      provides ready-to-use reagents for detailed analyses at the gene level, FISH 
      studies of chromosomal aberrations, and DNA sequencing.
FAU - Garcia, E
AU  - Garcia E
AD  - Human Genome Center, Lawrence Livermore National Laboratory, Livermore, 
      California 94550, USA.
FAU - Elliott, J
AU  - Elliott J
FAU - Gorvad, A
AU  - Gorvad A
FAU - Brandriff, B
AU  - Brandriff B
FAU - Gordon, L
AU  - Gordon L
FAU - Soliman, K M
AU  - Soliman KM
FAU - Ashworth, L K
AU  - Ashworth LK
FAU - Lennon, G
AU  - Lennon G
FAU - Burgin, M
AU  - Burgin M
FAU - Lamerdin, J
AU  - Lamerdin J
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosome Walking
MH  - *Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 19
MH  - Cosmids
MH  - Databases, Factual
MH  - Gene Library
MH  - Genetic Markers
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - S0888754385710075 [pii]
AID - 10.1006/geno.1995.1007 [doi]
PST - ppublish
SO  - Genomics. 1995 May 1;27(1):52-66. doi: 10.1006/geno.1995.1007.