PMID- 7665209
OWN - NLM
STAT- MEDLINE
DCOM- 19951006
LR  - 20190920
IS  - 0105-6263 (Print)
IS  - 0105-6263 (Linking)
VI  - 18
IP  - 2
DP  - 1995 Apr
TI  - Comparative analysis of proteins secreted in vitro by isolated seminiferous 
      tubules from man and the rat.
PG  - 103-11
AB  - The aims of this study were to provide an overall comparison of the in-vitro 
      protein secretory profile of seminiferous tubules (ST) isolated from man and the 
      rat, and to identify specific proteins secreted by both species. Two-dimensional 
      SDS PAGE was used to compare the profile of proteins secreted by cultured ST from 
      15 men undergoing orchidectomy with those secreted by ST isolated at stages 
      VI-VIII of the spermatogenic cycle from normal adult rats, and by ST at the same 
      stages isolated from rats pretreated with methoxyacetic acid (MAA) in order to 
      deplete both pachytene spermatocytes and round spermatids. Two abundant groups of 
      proteins not present in the medium of rat ST were secreted consistently by human 
      ST, though the profile of proteins secreted by human ST was otherwise more 
      variable than in the rat. Twelve proteins secreted by ST isolated from humans 
      were identified as possible homologues of rat ST-secreted proteins, including the 
      major rat Sertoli cell products SGP-1 and SGP-2, and an androgen-regulated 
      protein which derives in the rat from round spermatids. Otherwise, the majority 
      of the 12 potential homologues corresponded to proteins which in the rat are 
      secreted by ST from both normal and germ cell-depleted testes or by ST from germ 
      cell-depleted testes only, suggesting that they are probably secreted by Sertoli 
      cells and/or peritubular cells. Based on the potential homology of the proteins 
      identified, our results suggest, first, that these proteins may play an important 
      role in spermatogenesis and second, that the profile of proteins secreted by 
      human ST is more akin to that secreted by ST isolated from germ cell-depleted 
      rats.
FAU - McKinnell, C
AU  - McKinnell C
AD  - MRC Reproductive Biology Unit, Edinburgh, UK
FAU - Brackenbury, E T
AU  - Brackenbury ET
FAU - Qureshi, S J
AU  - Qureshi SJ
FAU - Hargreave, T B
AU  - Hargreave TB
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Blotting, Western
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Seminiferous Tubules/*metabolism
MH  - Species Specificity
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1111/j.1365-2605.1995.tb00394.x [doi]
PST - ppublish
SO  - Int J Androl. 1995 Apr;18(2):103-11. doi: 10.1111/j.1365-2605.1995.tb00394.x.