PMID- 7665230
OWN - NLM
STAT- MEDLINE
DCOM- 19951010
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 62
IP  - 5
DP  - 1995 Sep 4
TI  - Human peri-tumoral and lung fibroblasts produce paracrine motility factors for 
      recently established human sarcoma cell strains.
PG  - 585-92
AB  - Paracrine motogenic cytokines secreted by normal cells can stimulate metastatic 
      cell invasion. For example, human fibroblasts secrete hepatocyte growth 
      factor/scatter factor (HGF/SF), which stimulates paracrine migration of 
      epithelial and certain carcinoma cells, and migration-stimulating factor (MSF), 
      which stimulates autocrine migration of fibroblasts from certain breast 
      carcinomas. We found that human peri-tumoral and lung fibroblasts secrete 
      motility-stimulating activity for several recently established human sarcoma cell 
      strains. Motility of lung metastasis-derived SYN-I sarcoma cells was 
      preferentially stimulated by human lung and peri-tumoral fibroblast 
      motility-stimulating factors (FMSFs). FMSFs were non-dialyzable, susceptible to 
      trypsin and sensitive to dithiothreitol. Cycloheximide inhibited accumulation of 
      FMSF activity in conditioned medium; however, addition of cycloheximide to the 
      migration assay did not significantly affect motility-stimulating activity. 
      Purified HGF/SF, rabbit anti-hHGF and RT-PCR analysis of peri-tumoral and lung 
      fibroblast HGF/SF mRNA expression indicated that FMSF activity was unrelated to 
      HGF/SF. Partial purification of FMSF by gel exclusion chromatography revealed 
      several peaks of activity, suggesting multiple FMSF molecules or complexes. Since 
      human soft tissue sarcomas have a distinctive hematogenous metastatic pattern 
      (predominantly lung), FMSF may play a role in this process independent of HGF/SF.
FAU - Hu, M
AU  - Hu M
AD  - Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Pollock, R E
AU  - Pollock RE
FAU - Nakamura, T
AU  - Nakamura T
FAU - Nicolson, G L
AU  - Nicolson GL
LA  - eng
GR  - R35 CA44352/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA Primers)
RN  - 0 (Fibronectins)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (fibroblast motility-stimulating factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Base Sequence
MH  - *Cell Movement
MH  - DNA Primers/chemistry
MH  - Fibroblasts/physiology
MH  - Fibronectins/genetics
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/genetics
MH  - In Vitro Techniques
MH  - Lung/*cytology
MH  - Lung Neoplasms/secondary
MH  - Molecular Sequence Data
MH  - Peptide Fragments
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Sarcoma/*pathology
MH  - Tumor Cells, Cultured
EDAT- 1995/09/04 00:00
MHDA- 1995/09/04 00:01
CRDT- 1995/09/04 00:00
PHST- 1995/09/04 00:00 [pubmed]
PHST- 1995/09/04 00:01 [medline]
PHST- 1995/09/04 00:00 [entrez]
AID - 10.1002/ijc.2910620516 [doi]
PST - ppublish
SO  - Int J Cancer. 1995 Sep 4;62(5):585-92. doi: 10.1002/ijc.2910620516.