PMID- 7666433
OWN - NLM
STAT- MEDLINE
DCOM- 19951011
LR  - 20061115
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 252
IP  - 1
DP  - 1995 Sep 8
TI  - Requirements for DNA strand transfer during reverse transcription in mutant HIV-1 
      virions.
PG  - 59-69
AB  - Retroviruses convert their RNA genome into a DNA form by means of reverse 
      transcription. According to the current model of reverse transcription, two 
      strand transfer reactions are needed to synthesize a full-length DNA genome. 
      Because reverse transcription is initiated close to the 5' end of the RNA genome, 
      the first strand transfer translocates the minus-strand cDNA to the 3' end of the 
      viral genome. This jump is facilitated by the presence of a perfect repeat 
      element (R) at both ends of a retroviral genome. Strand transfer has been 
      extensively studied in in vitro systems with purified reverse transcriptase 
      enzyme (RT) and nucleic acid donor and acceptor templates. In this study, we set 
      out to test several parameters of the strand transfer reaction as it occurs in 
      cells infected with the human immunodeficiency virus (HIV-1). We constructed 
      mutant HIV-1 genomes with 3' R acceptor sequences that were specifically altered 
      either in length or structure. Analysis of the replication characteristics of the 
      mutant viruses indicates that repeats much shorter than the wild-type 
      97-nucleotides R region can efficiently act as acceptors during reverse 
      transcription. Furthermore, the introduction of excessively stable hairpin 
      structures within the 3' R element did only marginally affect the strand transfer 
      efficiency. We also analysed the DNA forms inherited upon infection of cells with 
      HIV-1 templates with multiple 3' R copies. These experiments indicate that 
      various 3' R repeats can serve as acceptor during minus-strand DNA transfer.
FAU - Berkhout, B
AU  - Berkhout B
AD  - Department of Virology, University of Amsterdam, The Netherlands.
FAU - van Wamel, J
AU  - van Wamel J
FAU - Klaver, B
AU  - Klaver B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers/chemistry
MH  - DNA, Viral/*biosynthesis/*metabolism
MH  - HIV-1/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - RNA, Viral/genetics
MH  - Transcription, Genetic
MH  - Virion
MH  - *Virus Replication
EDAT- 1995/09/08 00:00
MHDA- 1995/09/08 00:01
CRDT- 1995/09/08 00:00
PHST- 1995/09/08 00:00 [pubmed]
PHST- 1995/09/08 00:01 [medline]
PHST- 1995/09/08 00:00 [entrez]
AID - S0022-2836(84)70475-X [pii]
AID - 10.1006/jmbi.1994.0475 [doi]
PST - ppublish
SO  - J Mol Biol. 1995 Sep 8;252(1):59-69. doi: 10.1006/jmbi.1994.0475.