PMID- 766697
OWN - NLM
STAT- MEDLINE
DCOM- 19760423
LR  - 20170214
IS  - 0004-5632 (Print)
IS  - 0004-5632 (Linking)
VI  - 13
IP  - 1
DP  - 1976 Jan
TI  - An accurate method for the determination of inorganic phosphate in serum.
PG  - 306-12
AB  - Cali's approach to accuracy is reviewed, and it is concluded that the main factor 
      limiting its extension to analytes other than elements is the lack of suitable 
      definitive methods of analysis. The work published on calcium, andwork known to 
      be under way on other elements, is based on the use of stable isotope 
      dilution-mass spectrometry (I.D.M.S.) as the definitive technique. This paper 
      outliones the theorectical considerations involved in extending I.D.M.S. to the 
      determinatioons of molecules; in particular, exact equations for the calibration 
      graph obtained by I.D.M.S. are developed, and special cases of practical 
      significance are defined. It is concluded that in theory there is no reason why 
      accurate resluts should not be realised. Ther determination of isotope rations is 
      central to the tecnique of I.D.M.S. The means available for such measurments are 
      discussed, and it is concluded that adequate precision cnan be achieved with 
      standard equipment/ To illustrate the application for I.D.M.S. to molecules the 
      steps involved in an assay for inorganic phosphate are defined, and the protocol 
      this evolved is applied to specimens from the Wellcome Group Quality Control 
      Programme. Although good correlation was found between I.D.M.S. results and the 
      participants' mean results, there was a substantial bias. It is argued that this 
      is a result of the difficulty of defining "inorganic phosphate" as a chemical 
      entity. Some requirements for...
FAU - Pickup, J F
AU  - Pickup JF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - 0 (Oxygen Isotopes)
RN  - 0 (Phosphates)
RN  - 0 (Phosphorus Isotopes)
SB  - IM
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Indicator Dilution Techniques
MH  - Mass Spectrometry/methods
MH  - Oxygen Isotopes
MH  - Phosphates/*blood
MH  - Phosphorus Isotopes
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1177/000456327601300102 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 1976 Jan;13(1):306-12. doi: 10.1177/000456327601300102.