PMID- 7667907
OWN - NLM
STAT- MEDLINE
DCOM- 19951010
LR  - 20200505
IS  - 0378-1135 (Print)
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 44
IP  - 1
DP  - 1995 Apr
TI  - Antigenic differences between European and American isolates of porcine 
      reproductive and respiratory syndrome virus (PRRSV) are encoded by the 
      carboxyterminal portion of viral open reading frame 3.
PG  - 65-76
AB  - Antigenic differences between European and American isolates of porcine 
      reproductive and respiratory syndrome virus (PRRSV) were revealed by serologic 
      analysis of a recombinant protein derived from PRRSV open reading frame 3 (ORF 
      3). The hydrophilic carboxyterminal 199 amino acids encoded by the ORF 3 of a 
      European (Lelystad) isolate of PRRSV were expressed as a recombinant fusion 
      protein (BP03-P) in a baculovirus gene expression system. Sera from gnotobiotic 
      swine exposed to prototypic reference European and American isolates of PRRSV and 
      sera from conventionally reared European and American swine convalescing from 
      naturally acquired PRRSV infections were used to characterize the BP03-P protein. 
      Sera from gnotobiotic and conventionally reared swine exposed to European 
      isolates of PRRSV were significantly more reactive (P < 0.01) with BP03-P than 
      were the corresponding American PRRSV antisera using the indirect 
      immunoperoxidase monolayer assay (IPMA). Prototypic European, but not American, 
      PRRSV antisera also recognized BP03-P using western immunoblotting and 
      radioimmunoprecipitation assay (RIPA) procedures. However, gnotobiotically 
      derived antiserum to an atypical American-origin PRRSV was reactive with BP03-P 
      by both IPMA and western immunoblot. Despite a predicted potential for N-linked 
      glycosylation, studies with tunicamycin and peptide-N-glycosidase F (PNGase F) 
      indicated that BP03-P was not N-glycosylated in either insect cell cultures or 
      Trichoplusia ni larvae infected with the recombinant baculovirus. Sera from 
      rabbits inoculated with BP03-P failed to neutralize both the European (Lelystad) 
      and American (ATCC VR-2332) reference isolates of PRRSV and did not react by IPMA 
      with PRRSV-infected cell cultures. Taken together, the data suggest that the 
      carboxyterminal portion of PRRSV ORF 3 encodes a non-neutralizing viral peptide 
      that is partially responsible for the serologic differences noted between 
      European and most American isolates of PRRSV.
FAU - Katz, J B
AU  - Katz JB
AD  - U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Ames, 
      IA 50010, USA.
FAU - Shafer, A L
AU  - Shafer AL
FAU - Eernisse, K A
AU  - Eernisse KA
FAU - Landgraf, J G
AU  - Landgraf JG
FAU - Nelson, E A
AU  - Nelson EA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - *Antigenic Variation
MH  - Arterivirus/*genetics/immunology/*isolation & purification
MH  - Arterivirus Infections/*veterinary/virology
MH  - Baculoviridae/genetics/metabolism
MH  - Blotting, Western/methods
MH  - Europe
MH  - Female
MH  - Genes, Viral
MH  - Genital Diseases, Female/veterinary/virology
MH  - Immunoenzyme Techniques
MH  - North America
MH  - *Open Reading Frames
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Recombinant Proteins/analysis/biosynthesis
MH  - Respiratory Tract Diseases/veterinary/virology
MH  - Swine
MH  - *Swine Diseases
MH  - Syndrome
PMC - PMC7117291
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
PMCR- 2000/03/10
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PHST- 2000/03/10 00:00 [pmc-release]
AID - 037811359400113B [pii]
AID - 0378-1135(94)00113-B [pii]
AID - 10.1016/0378-1135(94)00113-b [doi]
PST - ppublish
SO  - Vet Microbiol. 1995 Apr;44(1):65-76. doi: 10.1016/0378-1135(94)00113-b.