PMID- 7668847
OWN - NLM
STAT- MEDLINE
DCOM- 19951006
LR  - 20190909
IS  - 0273-2289 (Print)
IS  - 0273-2289 (Linking)
VI  - 51-52
DP  - 1995 Spring
TI  - Screening of Bothrops snake venoms for L-amino acid oxidase activity.
PG  - 197-210
AB  - Toxins, enzymes, and biologically active peptides are the main components of 
      snake venoms from the genus Bothrops. Following the venom inoculation, the local 
      effects are hemorrhage, edema, and myonecrosis. Nineteen different species of 
      Brazilian Bothrops were screened for protein content and L-amino acid oxidase 
      activity. B. cotiara, formerly found in the South of Brazil, is now threatened 
      with extinction. Its venom contains a highly hemorrhagic fraction and, as 
      expected from the deep yellow color of the corresponding lyophilized powder, a 
      high L-amino acid oxidase (LAO) activity was also characterized. Flavin adenine 
      dinucleotide (FAD) is its associate coenzyme. B. cotiara venom LAO catalyzed the 
      oxidative deamination of several L-amino acids, and the best substrates were 
      methionine, leucine, tryptophan, and phenylalanine, hence, its potential 
      application for the use of biosensors for aspartame determination and for the 
      removal of amino acids from plasma. High levels for LAO were also found in other 
      species than B. cotiara. In addition, the technique of isoelectric focusing (IEF) 
      was employed as a powerful tool to study the iso- or multi-enzyme distribution 
      for LAO activity in the B. cotiara snake venom.
FAU - Pessatti, M
AU  - Pessatti M
AD  - Department of Biochemistry, UFPR-Federal University of Parana, Curitiba--PR, 
      Brazil.
FAU - Fontana, J D
AU  - Fontana JD
FAU - Furtado, M F
AU  - Furtado MF
FAU - Guimaraes, M F
AU  - Guimaraes MF
FAU - Zanette, L R
AU  - Zanette LR
FAU - Costa, W T
AU  - Costa WT
FAU - Baron, M
AU  - Baron M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Amino Acids)
RN  - 0 (Proteins)
RN  - 0 (Snake Venoms)
RN  - 146-14-5 (Flavin-Adenine Dinucleotide)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
EIN - Appl Biochem Biotechnol 1995 Dec;55(3):276
MH  - Amino Acid Oxidoreductases/chemistry/*metabolism
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Bothrops/genetics/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Dose-Response Relationship, Drug
MH  - Flavin-Adenine Dinucleotide/chemistry
MH  - Hemorrhage/chemically induced
MH  - Isoelectric Focusing
MH  - L-Amino Acid Oxidase
MH  - Proteins/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Snake Venoms/adverse effects/*chemistry
MH  - Spectrum Analysis
MH  - Zinc/chemistry
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF02933424 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 1995 Spring;51-52:197-210. doi: 10.1007/BF02933424.