PMID- 7669052
OWN - NLM
STAT- MEDLINE
DCOM- 19951012
LR  - 20181217
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 214
IP  - 1
DP  - 1995 Sep 5
TI  - An inhibitory role for phosphatidylinositol 3-kinase in insulin secretion from 
      pancreatic B cell line MIN6.
PG  - 51-9
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) has been implicated in the regulation 
      of vesicular transport. We examined the roles of PI3-kinase in the 
      glucose-induced insulin secretion from the pancreatic beta cell line MIN6 by 
      using wortmannin, a potent inhibitor of PI3-kinase. Low concentrations of 
      wortmannin markedly potentiated the glucose-induced insulin secretion. This 
      effect was probably mediated by PI3-kinase inhibition. Furthermore, wortmannin 
      completely canceled the suppressive effect of insulin-like growth factor-I on 
      insulin secretion from MIN6 cells. On the basis of these results, we discuss a 
      possible role of PI3-kinase in the negative feed-back regulation of insulin 
      secretion.
FAU - Hagiwara, S
AU  - Hagiwara S
AD  - Department of Disease-related Gene Regulation Research (Sandoz), Faculty of 
      Medicine, University of Tokyo, Japan.
FAU - Sakurai, T
AU  - Sakurai T
FAU - Tashiro, F
AU  - Tashiro F
FAU - Hashimoto, Y
AU  - Hashimoto Y
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Nonomura, Y
AU  - Nonomura Y
FAU - Miyazaki, J
AU  - Miyazaki J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Androstadienes)
RN  - 0 (Insulin)
RN  - 0 (Proteins)
RN  - 51110-01-1 (Somatostatin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/metabolism/pharmacology
MH  - Cell Line
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Islets of Langerhans/enzymology/*metabolism
MH  - Phosphatidylinositol 3-Kinases
MH  - Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors/*metabolism
MH  - Protein Binding
MH  - Proteins/metabolism
MH  - Somatostatin/pharmacology
MH  - Wortmannin
EDAT- 1995/09/05 00:00
MHDA- 1995/09/05 00:01
CRDT- 1995/09/05 00:00
PHST- 1995/09/05 00:00 [pubmed]
PHST- 1995/09/05 00:01 [medline]
PHST- 1995/09/05 00:00 [entrez]
AID - S0006-291X(85)72255-3 [pii]
AID - 10.1006/bbrc.1995.2255 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Sep 5;214(1):51-9. doi: 10.1006/bbrc.1995.2255.