PMID- 7670643
OWN - NLM
STAT- MEDLINE
DCOM- 19951013
LR  - 20210402
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 141 ( Pt 6)
DP  - 1995 Jun
TI  - Discovery of a ptsHI operon, which includes a third gene (ptsT), in the 
      thermophile Bacillus stearothermophilus.
PG  - 1443-1449
LID - 10.1099/13500872-141-6-1443 [doi]
AB  - The discovery of ptsHI operon in Bacillus stearothermophilus XL-65-6 coupled with 
      our previous report of a cel operon (Lai & Ingram, J Bacteriol 175, 6441-6450, 
      1993) demonstrates that this thermophilic organism contains all of the genes 
      required for cellobiose uptake by the phosphoenolpyruvate-dependent 
      phosphotransferase system (PTS). Genes encoding the two general PTS proteins, HPr 
      (ptsH) and enzyme I (ptsI), were cloned and sequenced. These form an operon which 
      includes a third small gene (ptsT) of unknown function (encoded product M(r) 
      18428). Both ptsH and ptsI were expressed at high levels from a single plasmid in 
      Escherichia coli and complemented corresponding host mutations. Although the 
      translated sequences for these genes were similar to homologues from 
      Gram-positive mesophiles (64-77% identity), the B. stearothermophilus gene 
      products were unusual in having a higher predicted pI and fewer negatively 
      charged amino acid residues. Enzyme I also contained more alanine and leucine 
      than mesophilic counterparts. Interestingly, ptsT inhibited the growth of E. coli 
      ptsI mutants at 37 degrees C. No such inhibition was observed during incubation 
      at a lower temperature (30 degrees C) or in E. coli DH5 alpha, which is wild-type 
      for ptsI. The predicted translation product from ptsT contained a high proportion 
      of basic amino acids (27%) and had a high predicted pI (pH 11.7), properties 
      similar to bacterial histone-like proteins, but did not exhibit homology to any 
      sequences in the current database. Regions upstream and downstream from the ptsHI 
      operon contain genes with homology to Bacillus subtilis ptsG and wapA 
      (wall-associated protein), respectively.
FAU - Lai, Xiaokuang
AU  - Lai X
AD  - Department of Microbiology and Cell Science, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
FAU - Ingram, Lonnie O
AU  - Ingram LO
AD  - Department of Microbiology and Cell Science, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
LA  - eng
SI  - GENBANK/U12340
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
RN  - 0 (PTST protein, Bacillus stearothermophilus)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.1.- (Phosphoenolpyruvate Sugar Phosphotransferase System)
RN  - EC 2.7.1.- (phosphocarrier protein HPr)
RN  - EC 2.7.3.- (Phosphotransferases (Nitrogenous Group Acceptor))
RN  - EC 2.7.3.9 (phosphoenolpyruvate-protein phosphotransferase)
SB  - IM
GS  - ptsG
GS  - ptsH
GS  - ptsI
GS  - ptsT
GS  - wapA
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/biosynthesis/*genetics
MH  - Base Sequence
MH  - Escherichia coli
MH  - Frameshift Mutation
MH  - *Genes, Bacterial
MH  - Geobacillus stearothermophilus/*genetics
MH  - Molecular Sequence Data
MH  - *Operon
MH  - Phosphoenolpyruvate Sugar Phosphotransferase System/biosynthesis/*genetics
MH  - Phosphotransferases (Nitrogenous Group Acceptor)/biosynthesis/*genetics
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Temperature
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1099/13500872-141-6-1443 [doi]
PST - ppublish
SO  - Microbiology (Reading). 1995 Jun;141 ( Pt 6):1443-1449. doi: 
      10.1099/13500872-141-6-1443.