PMID- 7672000
OWN - NLM
STAT- MEDLINE
DCOM- 19951018
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 278
IP  - 2
DP  - 1995 May 15
TI  - Effect of azelastine on airway hyperresponsiveness mediated by stimulated 
      macrophages.
PG  - 161-6
AB  - The effect of the anti-allergic drug azelastine, 
      4-(p-chlorobenzyl)-2-(hexahydro-1-methyl-1H-azepine-4-yl)-1-(2H)-phth alazione), 
      on airway hyperresponsiveness induced by immunologically stimulated pulmonary 
      alveolar macrophages was investigated in canine bronchial segments under 
      isometric conditions in vitro. Macrophages stimulated with anti-dinitrophenyl 
      immunoglobulin E (IgE) antibody and dinitrophenyl-human serum albumin potentiated 
      the contractile responses to electrical field stimulation at all frequencies, an 
      effect that was abolished by azelastine (3 x 10(-5) M). In contrast, azelastine 
      had no effect on the potentiation of the contractile responses to electrical 
      stimulation by U46619, a thromboxane A2 mimetic. The IgE-mediated release of 
      thromboxane A2 from macrophages was inhibited by azelastine in a 
      concentration-dependent fashion, the maximal decrease and the concentration 
      required to produce a half-maximal effect being 84 +/- 6% (P < 0.001) and 16 
      microM, respectively. These results suggest that azelastine may attenuate 
      macrophage-induced parasympathetic airway hyperresponsiveness through an 
      inhibition of the release of thromboxane A2.
FAU - Tamaoki, J
AU  - Tamaoki J
AD  - First Department of Medicine, Tokyo Women's Medical College, Japan.
FAU - Takemura, H
AU  - Takemura H
FAU - Tagaya, E
AU  - Tagaya E
FAU - Sakai, A
AU  - Sakai A
FAU - Yamawaki, I
AU  - Yamawaki I
FAU - Konno, K
AU  - Konno K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Histamine H1 Antagonists)
RN  - 0 (Phthalazines)
RN  - 0 (Prostaglandin Endoperoxides, Synthetic)
RN  - 0 (Serum Albumin)
RN  - 0 (Vasoconstrictor Agents)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 57576-52-0 (Thromboxane A2)
RN  - 76898-47-0 (15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid)
RN  - ZQI909440X (azelastine)
SB  - IM
MH  - 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
MH  - Animals
MH  - Bronchial Hyperreactivity/*physiopathology
MH  - Dogs
MH  - Electric Stimulation
MH  - Female
MH  - Histamine H1 Antagonists/*pharmacology
MH  - Immunoglobulin E/immunology
MH  - In Vitro Techniques
MH  - Isometric Contraction/drug effects
MH  - Macrophage Activation/drug effects
MH  - Macrophages, Alveolar/*physiology
MH  - Male
MH  - Parasympathetic Nervous System/drug effects
MH  - Phthalazines/*pharmacology
MH  - Prostaglandin Endoperoxides, Synthetic/pharmacology
MH  - Serum Albumin/immunology
MH  - Synaptic Transmission/drug effects
MH  - Thromboxane A2/analogs & derivatives/metabolism/pharmacology
MH  - Vasoconstrictor Agents/pharmacology
EDAT- 1995/05/15 00:00
MHDA- 1995/05/15 00:01
CRDT- 1995/05/15 00:00
PHST- 1995/05/15 00:00 [pubmed]
PHST- 1995/05/15 00:01 [medline]
PHST- 1995/05/15 00:00 [entrez]
AID - 0014-2999(95)00114-Z [pii]
AID - 10.1016/0014-2999(95)00114-z [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1995 May 15;278(2):161-6. doi: 10.1016/0014-2999(95)00114-z.