PMID- 7673718
OWN - NLM
STAT- MEDLINE
DCOM- 19951016
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 155
IP  - 6
DP  - 1995 Sep 15
TI  - The identification and cloning of a murine major basic protein gene expressed in 
      eosinophils.
PG  - 3002-12
AB  - The existence of a murine homologue of the major basic protein (MBP) found in 
      human eosinophil granules was initially hypothesized from structural similarities 
      at the electron microscopic level. The results presented in this study have 
      extended these observations by describing the identification/purification of a 
      mouse MBP (mMBP) and the cloning of the gene encoding this eosinophil granule 
      protein. Using protein purification methodologies with extravascular eosinophils, 
      an mMBP homologue has been identified on the basis of strong (64%) N-terminal 
      sequence homology with the mature human MBP (hMBP). Since hMBP results from a 
      proteolytic cleavage of a precursor molecule, this sequence conservation suggests 
      that the mouse granule protein is processed by a similar mechanism. The gene 
      encoding mMBP was isolated using a hMBP cDNA clone as a heterologous probe in low 
      criteria screens of mouse genomic and cDNA libraries. The genomic structure and 
      nucleotide sequence of the mMBP exons are well conserved with the human gene, 
      although homology alignments of the encoded proteins show that extensive sequence 
      conservation occurs only in the mature portion of the MBP molecules. Expression 
      data demonstrate that this gene is transcriptionally active in tissues containing 
      eosinophil progenitor cells, such as femoral bone marrow. Genomic Southern blots 
      using the mMBP gene at reduced stringency reveal the potential existence of a 
      second, more divergent MBP-like sequence in the mouse. This suggests that, as 
      with guinea pigs, the mouse genome may also encode the eosinophil major basic 
      protein from more than one gene.
FAU - Larson, K A
AU  - Larson KA
AD  - Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, AZ 
      85259, USA.
FAU - Horton, M A
AU  - Horton MA
FAU - Madden, B J
AU  - Madden BJ
FAU - Gleich, G J
AU  - Gleich GJ
FAU - Lee, N A
AU  - Lee NA
FAU - Lee, J J
AU  - Lee JJ
LA  - eng
SI  - GENBANK/L46768
GR  - AI09728/AI/NIAID NIH HHS/United States
GR  - T32AI07047/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Blood Proteins)
RN  - 0 (Eosinophil Granule Proteins)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood Proteins/*genetics/*isolation & purification
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Eosinophil Granule Proteins
MH  - Eosinophils/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Ribonucleases
MH  - Sequence Alignment
EDAT- 1995/09/15 00:00
MHDA- 1995/09/15 00:01
CRDT- 1995/09/15 00:00
PHST- 1995/09/15 00:00 [pubmed]
PHST- 1995/09/15 00:01 [medline]
PHST- 1995/09/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Sep 15;155(6):3002-12.