PMID- 7677927
OWN - NLM
STAT- MEDLINE
DCOM- 19921222
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 295
IP  - 1
DP  - 1993 Jan
TI  - Enhancement of age-related increases in DNA I-compound levels by calorie 
      restriction: comparison of male B-N and F-344 rats.
PG  - 31-46
AB  - Caloric restriction (CR), known to extend median and maximum life spans, improve 
      resistance to carcinogenesis, and significantly retard age-associated 
      degenerative diseases in rodents, was previously reported to modulate levels of 
      indigenous, age-dependent DNA modifications, called I-compounds, in male 
      Brown-Norway (B-N) rats. Since profiles of these adduct-like derivatives are 
      species-, strain-, sex-, and tissue-specific, we explored this apparent 
      CR/I-compound relationship in a comparative study between male B-N and male 
      Fischer 344 (F-344) rats, the latter having a shorter life expectancy and high 
      incidence of renal disease. Control animals were fed NIH-31 diet ad libitum (AL), 
      while the caloric intake of CR animals was limited to 60% of AL, starting at 3.5 
      months. Liver and kidney DNA from 1, 8, 12, 16, 24 (AL, CR), and 30 (CR only) 
      month old rats was analyzed by 32P-postlabeling. Corresponding tissues from the 
      two strains yielded similar DNA profiles. Total liver I-compound levels displayed 
      2.3-4.6-fold age-dependent increases from 1 to 24 months, and kidney values at 24 
      months were 5.2-8 times higher than those at 1 month. In both strains, I-compound 
      levels of CR animals were higher, up to 2-fold, than in age-matched AL rats. 
      Regression analyses indicated linear relationships between most CR relative 
      adduct labeling values (both total and individual fractions) and age, whereas 
      many AL values exhibited this type of link with log age. These findings confirm 
      that a correlation exists between CR and I-compound levels, and, given the above 
      physiological benefits of CR, indicate that I-compounds represent biomarkers of 
      aging with potential utility in intervention studies.
FAU - Randerath, K
AU  - Randerath K
AD  - Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
FAU - Hart, R W
AU  - Hart RW
FAU - Zhou, G D
AU  - Zhou GD
FAU - Reddy, R
AU  - Reddy R
FAU - Danna, T F
AU  - Danna TF
FAU - Randerath, E
AU  - Randerath E
LA  - eng
GR  - R01 AG 07750/AG/NIA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - DNA/*metabolism
MH  - *DNA Damage
MH  - Energy Intake
MH  - *Food Deprivation
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 0921-8734(93)90009-R [pii]
AID - 10.1016/0921-8734(93)90009-r [doi]
PST - ppublish
SO  - Mutat Res. 1993 Jan;295(1):31-46. doi: 10.1016/0921-8734(93)90009-r.