PMID- 7678973
OWN - NLM
STAT- MEDLINE
DCOM- 19930305
LR  - 20171116
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 9
IP  - 1
DP  - 1993 Jan
TI  - CD4+ human immunodeficiency virus type 1 (HIV-1) envelope-specific cytotoxic T 
      lymphocytes derived from the peripheral blood cells of an HIV-1-infected 
      individual.
PG  - 61-8
AB  - Virus-specific cytotoxic T lymphocytes (CTL) are frequently of the CD8+ surface 
      phenotype, although CTL of the CD4+ surface phenotype have also been described. 
      Published reports of CTL derived from peripheral blood mononuclear cells (PBMC) 
      of individuals infected with human immunodeficiency virus type 1 (HIV-1) have 
      described primarily cells of the CD8+ surface phenotype. However, CD4+ HIV-1 
      envelope-specific CTL have been reported after in vitro stimulation with HIV-1 
      envelope protein of peripheral blood cells obtained from HIV-1-seronegative 
      donors, in peripheral blood cells after vaccination of HIV-1-seronegative persons 
      with HIV-1 envelope proteins, and in cerebrospinal fluid cells of HIV-1-infected 
      individuals. Recently, CD4+ HIV-1 gag-specific CTL were also reported. We now 
      report a patient from whom we derived HIV-1 envelope-specific CTL cell lines of 
      the CD4+ surface phenotype. Our cell culture technique did not employ exogenous 
      viral antigenic stimulation, and may therefore yield cells that more closely 
      reflect those in the underlying populations from which they were derived. These 
      CTL did not appear to have the clear human leukocyte antigen (HLA) class II 
      restriction pattern typically seen in CD4-expressing cells and were not 
      functionally inhibited by anti-CD3 antibody. Further work will be required to 
      define the role of CD4+ CTL in the pathogenesis of HIV-1 disease.
FAU - Curiel, T J
AU  - Curiel TJ
AD  - Department of Medicine, University of Colorado Health Sciences Center, Denver 
      80262.
FAU - Wong, J T
AU  - Wong JT
FAU - Gorczyca, P F
AU  - Gorczyca PF
FAU - Schooley, R T
AU  - Schooley RT
FAU - Walker, B D
AU  - Walker BD
LA  - eng
GR  - CA 37461-07/CA/NCI NIH HHS/United States
GR  - R29-AI27050/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (CD4 Antigens)
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, env)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - CD4 Antigens/*analysis
MH  - Cell Line
MH  - Epitopes
MH  - Gene Products, env/*immunology
MH  - HIV Infections/*immunology
MH  - HIV-1/*immunology
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Male
MH  - Phenotype
MH  - T-Lymphocyte Subsets
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1089/aid.1993.9.61 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1993 Jan;9(1):61-8. doi: 10.1089/aid.1993.9.61.