PMID- 7678986
OWN - NLM
STAT- MEDLINE
DCOM- 19930308
LR  - 20220409
IS  - 1049-8834 (Print)
IS  - 1049-8834 (Linking)
VI  - 13
IP  - 2
DP  - 1993 Feb
TI  - An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear 
      leukocyte adhesion molecule, in rabbit aortic endothelium.
PG  - 197-204
AB  - Accumulation of monocyte-derived foam cells in focal areas of the arterial intima 
      is a key step in early atherogenesis. We investigated the expression of vascular 
      cell adhesion molecule-1 (VCAM-1), a mononuclear leukocyte adhesion molecule, in 
      the arterial endothelium during the early phases of diet-induced atherogenesis in 
      rabbits in vivo and the regulation of VCAM-1 expression by cytokines in rabbit 
      aortic organoid cultures in vitro. Rabbits were fed either an atherogenic diet 
      (containing 0.3% cholesterol, 9% coconut oil, and 1% corn oil) or an isocaloric 
      control diet (10% corn oil) for 4 days or 1, 3, 6, or 13 weeks. The endothelium 
      in the ascending aorta focally expressed VCAM-1 after only 1 week on the 
      atherogenic diet but before the first appearance of intimal macrophages, as 
      judged by immunohistochemical staining of serial sections. The rabbits that 
      consumed the atherogenic diet for 3 weeks or longer developed lesions in the 
      intima composed of macrophages bearing class II major histocompatibility antigen 
      (MHC-II). Endothelial cells continued to focally express VCAM-1 at sites of 
      MHC-II-positive intimal macrophages for up to 13 weeks. The ascending aortas of 
      control rabbits lacked VCAM-1 or MHC-II-positive endothelium or macrophages at 
      all times studied. These observations demonstrate the focal activation of 
      arterial endothelium as early as 7 days after initiation of an atherogenic diet 
      (at serum cholesterol levels of 308 +/- 57 mg/dl).(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Li, H
AU  - Li H
AD  - Vascular Medicine and Atherosclerosis Unit, Brigham and Women's Hospital, Boston, 
      MA 02115.
FAU - Cybulsky, M I
AU  - Cybulsky MI
FAU - Gimbrone, M A Jr
AU  - Gimbrone MA Jr
FAU - Libby, P
AU  - Libby P
LA  - eng
GR  - HL-34636/HL/NHLBI NIH HHS/United States
GR  - P01-HL-36028/HL/NHLBI NIH HHS/United States
GR  - R01-HL-45563/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arterioscler Thromb
JT  - Arteriosclerosis and thrombosis : a journal of vascular biology
JID - 9101388
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Triglycerides)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Aorta/metabolism
MH  - Arteriosclerosis/etiology/metabolism
MH  - Cell Adhesion Molecules/*biosynthesis
MH  - Cholesterol/blood
MH  - Cytokines/*pharmacology
MH  - *Diet, Atherogenic
MH  - Endothelium, Vascular/*metabolism
MH  - Male
MH  - Organ Culture Techniques
MH  - Rabbits
MH  - Triglycerides/blood
MH  - Vascular Cell Adhesion Molecule-1
EDAT- 1993/02/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1161/01.atv.13.2.197 [doi]
PST - ppublish
SO  - Arterioscler Thromb. 1993 Feb;13(2):197-204. doi: 10.1161/01.atv.13.2.197.