PMID- 7680298
OWN - NLM
STAT- MEDLINE
DCOM- 19930405
LR  - 20190512
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 91
IP  - 3
DP  - 1993 Mar
TI  - Primary structure of a variable region of the V kappa I subgroup (ISE) in light 
      chain deposition disease.
PG  - 506-9
AB  - Although structural abnormalities of monoclonal immunoglobulin light chains (LC) 
      are suspected to play a determinant role in non-amyloid light chain deposition 
      disease (LCDD), this condition is as yet poorly documented at the molecular 
      level, since only three sequences have been reported to date. In a case of 
      myeloma-associated LCDD, the patient's urine contained an unglycosylated kappa 
      Bence Jones protein made up of dimers and monomers with an apparent molecular 
      mass of 25,000 which was assigned to the V kappa I subgroup by N-terminal amino 
      acid sequencing. The complete variable region sequence of the monoclonal kappa 
      chain produced by the malignant plasma cells was amplified by polymerase chain 
      reaction (PCR) using small amounts of material obtained by bone marrow 
      aspiration. The sequence of three independently amplified cDNA clones derived 
      from a normal-sized kappa messenger RNA was identical to that of the urinary 
      kappa chain. The kappa mRNA had an overall normal structure made up of a V kappa 
      I sequence rearranged to J kappa I. Several unusual features of the variable 
      region (the first complete V kappa I sequence reported in LCDD) included three 
      substitutions that introduced hydrophobic residues at spatially close positions. 
      The strategy associating N-terminal sequence determination and cDNA cloning by 
      PCR could help in accumulating new sequence data and improving our understanding 
      of LCDD pathogenesis.
FAU - Rocca, A
AU  - Rocca A
AD  - CNRS URA 1172, Laboratory of Molecular Immunology, Faculty of Sciences and 
      University Hospital, Poitiers, France.
FAU - Khamlichi, A A
AU  - Khamlichi AA
FAU - Aucouturier, P
AU  - Aucouturier P
FAU - Noel, L H
AU  - Noel LH
FAU - Denoroy, L
AU  - Denoroy L
FAU - Preud'homme, J L
AU  - Preud'homme JL
FAU - Cogne, M
AU  - Cogne M
LA  - eng
SI  - GENBANK/X67322
SI  - GENBANK/X71069
SI  - GENBANK/X71070
SI  - GENBANK/X71071
SI  - GENBANK/X71675
SI  - GENBANK/X71676
SI  - GENBANK/X71677
SI  - GENBANK/X71678
SI  - GENBANK/X71679
SI  - GENBANK/X71680
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin kappa-Chains)
RN  - 63231-63-0 (RNA)
RN  - 9006-99-9 (Bence Jones Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Bence Jones Protein/genetics
MH  - Bone Marrow
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunoglobulin G/*genetics
MH  - Immunoglobulin kappa-Chains/*genetics
MH  - Molecular Sequence Data
MH  - Multiple Myeloma/genetics
MH  - Paraproteinemias/*genetics
MH  - Polymerase Chain Reaction
MH  - RNA/genetics
PMC - PMC1554718
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
PMCR- 1994/03/01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
PHST- 1994/03/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.1993.tb05932.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1993 Mar;91(3):506-9. doi: 10.1111/j.1365-2249.1993.tb05932.x.