PMID- 7680837
OWN - NLM
STAT- MEDLINE
DCOM- 19930415
LR  - 20190904
IS  - 0365-5237 (Print)
IS  - 0365-5237 (Linking)
VI  - 500
DP  - 1993
TI  - Experimental and clinical evaluation of DNA ploidy with use of double staining of 
      keratin/DNA by flow cytometer in head and neck tumor.
PG  - 149-53
AB  - As a basic study, an analysis was made, using flow cytometry (FCM), of a mixture 
      of KB cells and lymphocytes after Propidium Iodide (PI)- Fluorescein 
      Isothiocyanate (FITC) double strain. As a result, DNA histogram of only KB cells 
      could be drawn. As a clinical application an analysis was made, using FCM, of 
      tumor of the head and neck after double stain. It enabled the drawing of DNA 
      histogram of only epithelial cells (cancer cells). Changes in DNA histogram of 
      keratin positive cells were classified into three types: Type I: the same diploid 
      type as whole cells, Type II: the same aneuploid type as them, and Type III: the 
      different aneuploid type as them. Among 22 cases of malignant tumor there were 3 
      cases of Type I, 6 cases of Type II, and 13 cases of Type III. This method could 
      contribute greatly to the detection of cancer cells and DNA diagnosis, and thus 
      be useful clinically.
FAU - Yamazaki, N
AU  - Yamazaki N
AD  - Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
FAU - Kawamoto, K
AU  - Kawamoto K
FAU - Hori, Y
AU  - Hori Y
FAU - Kumazawa, H
AU  - Kumazawa H
FAU - Yamashita, T
AU  - Yamashita T
FAU - Kumazawa, T
AU  - Kumazawa T
LA  - eng
PT  - Journal Article
PL  - Norway
TA  - Acta Otolaryngol Suppl
JT  - Acta oto-laryngologica. Supplementum
JID - 0370355
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Squamous Cell/*pathology
MH  - DNA, Neoplasm/*analysis
MH  - Epithelium/pathology
MH  - Fibroblasts/pathology
MH  - *Flow Cytometry
MH  - Head and Neck Neoplasms/*pathology
MH  - Humans
MH  - Keratins/*analysis
MH  - Leukocyte Count
MH  - Lymphocytes/pathology
MH  - *Ploidies
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.3109/00016489309126200 [doi]
PST - ppublish
SO  - Acta Otolaryngol Suppl. 1993;500:149-53. doi: 10.3109/00016489309126200.