PMID- 7680917
OWN - NLM
STAT- MEDLINE
DCOM- 19930416
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 81
IP  - 6
DP  - 1993 Mar 15
TI  - Granulocyte colony-stimulating factor induces hFc gamma RI (CD64 
      antigen)-positive neutrophils via an effect on myeloid precursor cells.
PG  - 1457-64
AB  - In this study we have examined hFc gamma RI expression during myelopoiesis. 
      Normal bone marrow (BM) cells were found to express hFc gamma RI up to the 
      metamyelocyte stage. A different Fc gamma RI expression pattern was observed in 
      an in vitro model of myelopoiesis. Purified CD34-positive BM cells, cultured for 
      12 to 14 days with granulocyte colony-stimulating factor (G-CSF), differentiate 
      into a population of mature granulocytic cells. In these cultures, in which hFc 
      gamma RI was virtually absent on the initial CD34-positive BM cells, hFc gamma RI 
      was strongly induced by G-CSF after only 5 days. During final maturation the 
      cells remained hFc gamma RI positive. This expression was confirmed functionally 
      by antibody-sensitized erythrocytes (EA)-rosette assays. Moreover, the mature 
      myeloid cells were found to express mRNA encoding for hFc gamma RI, whereas 
      reverse-transcriptase polymerase chain reaction analysis showed that both hFc 
      gamma RIA and hFc gamma RIB genes were expressed. In contrast, on peripheral 
      blood (PB) polymorphonuclear neutrophil leukocytes (PMN) the in vitro effect of 
      G-CSF as to hFc gamma RI induction was limited. Therefore, we conclude that, with 
      respect to hFc gamma RI expression on PMN, G-CSF acts on myeloid precursor cells 
      rather than on mature cells. This conclusion could be strengthened by in vivo 
      administration of a single dose of G-CSF to a healthy volunteer. After a 12-hour 
      lag time, hFc gamma RI expressing PMNs were detected in the peripheral blood. 
      This study shows that hFc gamma RI is an early myeloid differentiation marker 
      that is lost during normal final maturation. However, committed myeloid 
      progenitor cells can be strongly induced by G-CSF to express hFc gamma RI, 
      ultimately resulting in mature granulocytic cells expressing the high-affinity 
      receptor for IgG. This expression may have important consequences for the 
      functional capacity of these cells.
FAU - Kerst, J M
AU  - Kerst JM
AD  - Central Laboratory of the Red Cross Blood Transfusion Service, Amsterdam, The 
      Netherlands.
FAU - van de Winkel, J G
AU  - van de Winkel JG
FAU - Evans, A H
AU  - Evans AH
FAU - de Haas, M
AU  - de Haas M
FAU - Slaper-Cortenbach, I C
AU  - Slaper-Cortenbach IC
FAU - de Wit, T P
AU  - de Wit TP
FAU - von dem Borne, A E
AU  - von dem Borne AE
FAU - van der Schoot, C E
AU  - van der Schoot CE
FAU - van Oers, R H
AU  - van Oers RH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Receptors, IgG)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, CD34
MH  - Base Sequence
MH  - *Bone Marrow Cells
MH  - Cells, Cultured
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*drug effects/physiology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neutrophils/*drug effects/immunology
MH  - Receptors, IgG/*analysis
EDAT- 1993/03/15 00:00
MHDA- 1993/03/15 00:01
CRDT- 1993/03/15 00:00
PHST- 1993/03/15 00:00 [pubmed]
PHST- 1993/03/15 00:01 [medline]
PHST- 1993/03/15 00:00 [entrez]
AID - S0006-4971(20)74692-6 [pii]
PST - ppublish
SO  - Blood. 1993 Mar 15;81(6):1457-64.