PMID- 7683198
OWN - NLM
STAT- MEDLINE
DCOM- 19930528
LR  - 20151119
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 8
IP  - 5
DP  - 1993 May
TI  - Phenotypic marker expression during fetal and neonatal differentiation of rat 
      tracheal epithelial cells.
PG  - 546-55
AB  - The expression of phenotypic markers was examined during fetal and neonatal 
      differentiation of rat tracheal epithelial (RTE) cells. The rat counterpart of 
      human keratin 18 was predominantly found in columnar cells in the adult trachea. 
      It was detected in the primordial tracheal epithelium first seen on gestational 
      day (GD) 12 (term = 21.5 days). Staining intensity gradually increased, and by GD 
      17 it was principally localized to the apical portion of the epithelium. The rat 
      counterpart of human keratin 19 was barely detectable in the trachea on GD 13 but 
      became abundant in almost all RTE cells on and after GD 19. Morphologically and 
      immunocytochemically identifiable secretory and ciliated cells appeared on GD 18. 
      Ciliated cell number slowly rose while secretory cells increased dramatically on 
      GD 19 through postnatal day 1. The secretory granule antigens detected by 
      monoclonal antibodies RTE 9 and 11 were rare in the adult trachea but were highly 
      expressed in virtually all of the perinatal secretory cells. In contrast, the 
      epitope detected by monoclonal antibody RTE 12, which was present in all adult 
      tracheal surface secretory cells, did not appear until postnatal day 1 and slowly 
      increased. These results demonstrate marked shifts in the biochemical composition 
      of secretory cells during development and postnatal maturation. For the 
      above-mentioned molecules, a similar expression pattern was observed during 
      epithelial regeneration in tracheal grafts (Am. J. Respir. Cell Mol. Biol. 1992; 
      7:30-41). Pseudo-stratification of the epithelium and basal cells was first 
      observed on GD 20. Keratin 14, which is confined to basal cells in the normal 
      adult trachea, was not present in the nascent basal cells but appeared after 
      postnatal day 1. In contrast to the present results, during epithelial 
      regeneration in tracheal grafts keratin 14 appeared before markers of highly 
      differentiated secretory or ciliated cells. Thus, the biochemical sequence of 
      cellular differentiation during regeneration did not precisely recapitulate 
      development.
FAU - Randell, S H
AU  - Randell SH
AD  - Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health 
      Sciences, Research Triangle Park, NC 27709.
FAU - Shimizu, T
AU  - Shimizu T
FAU - Bakewell, W
AU  - Bakewell W
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Nettesheim, P
AU  - Nettesheim P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers)
RN  - 68238-35-7 (Keratins)
RN  - P4448TJR7J (Alcian Blue)
SB  - IM
EIN - Am J Respir Cell Mol Biol 1993 Aug;9(2):following 229
MH  - Alcian Blue
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies, Monoclonal/immunology
MH  - Biomarkers
MH  - Cell Differentiation
MH  - Epithelial Cells
MH  - Female
MH  - Immunohistochemistry
MH  - Keratins/biosynthesis/immunology
MH  - Periodic Acid-Schiff Reaction
MH  - Phenotype
MH  - Pregnancy
MH  - Rats
MH  - Rats, Inbred F344
MH  - Trachea/*cytology/embryology/metabolism
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 10.1165/ajrcmb/8.5.546 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1993 May;8(5):546-55. doi: 10.1165/ajrcmb/8.5.546.