PMID- 7684023
OWN - NLM
STAT- MEDLINE
DCOM- 19930615
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 24
IP  - 5
DP  - 1993 May
TI  - Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions 
      by an anti-monocyte chemoattractant protein-1 monoclonal antibody.
PG  - 534-9
AB  - The infiltration of blood monocytes into the subendothelial space is thought to 
      be one of the most important pathologic events in early atherogenesis. To examine 
      the mechanism of monocyte migration in early atherosclerotic lesions we 
      investigated immunohistochemically the production of monocyte chemoattractant 
      protein-1 (MCP-1) in various atherosclerotic lesions, including diffuse intimal 
      thickening, fatty streaks, and atheromatous plaques, obtained during autopsies of 
      patients of various ages. A highly specific anti-MCP-1 monoclonal antibody that 
      does not cross-react with neutrophil-activating, attractant 
      protein-1/interleukin-8 or platelet proteins that have an amino acid sequence 
      similar to MCP-1 was used to localize MCP-1 in situ. To characterize the cells 
      constituting the atherosclerotic lesions a panel of monoclonal and polyclonal 
      antibodies that are specific to smooth muscle cells (HHF-35), 
      monocyte/macrophages (HAM56, Leu-M3, Leu-M5, EBM11, and PM-2K), and endothelial 
      cells (anti-von Willebrand factor) was used. Double immunohistochemical staining 
      with anti-MCP-1 and one of the cell type-specific antibodies was performed to 
      identify the nature of MCP-1-positive cells. Endothelial cells stained positively 
      for MCP-1 in nine of 14 diffuse intimal thickening lesions. Scattered macrophages 
      in thickened intima also were positive for MCP-1. Endothelial staining of MCP-1 
      was observed in 14 of 21 fatty streak lesions. Subendothelial macrophages were 
      strongly stained for MCP-1 in all fatty streak lesions examined. Subendothelial 
      macrophages were stained for MCP-1 in atherosclerotic plaques; however, 
      endothelial cells were only slightly positive for MCP-1. A few smooth muscle 
      cells in the intima were positive for MCP-1 in atheromatous plaques. From these 
      results it is concluded that the cell populations positive for MCP-1 are 
      different in early and advanced atherosclerotic lesions, and that the endothelial 
      cells and subendothelial macrophages are considered to be the major sources of 
      MCP-1 in early atherosclerotic lesions.
FAU - Takeya, M
AU  - Takeya M
AD  - Second Department of Pathology, Kumamoto University School of Medicine, Japan.
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Leonard, E J
AU  - Leonard EJ
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Monoclonal/*immunology
MH  - Arteriosclerosis/*metabolism/pathology
MH  - Chemokine CCL2
MH  - Chemotactic Factors/immunology/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Staining and Labeling
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 0046-8177(93)90166-E [pii]
AID - 10.1016/0046-8177(93)90166-e [doi]
PST - ppublish
SO  - Hum Pathol. 1993 May;24(5):534-9. doi: 10.1016/0046-8177(93)90166-e.