PMID- 7684192
OWN - NLM
STAT- MEDLINE
DCOM- 19930614
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 142
IP  - 5
DP  - 1993 May
TI  - Detection of N-myc gene amplification by fluorescence in situ hybridization. 
      Diagnostic utility for neuroblastoma.
PG  - 1339-46
AB  - We assessed fluorescence in situ hybridization (FISH) as an alternative to 
      Southern blot analysis for determination of N-myc gene amplification in 
      neuroblastoma. In the 44 pediatric solid tumor cell lines examined (20 
      neuroblastomas), the mean number of N-myc copies determined by FISH correlated 
      closely with Southern blot results. There was wide intercellular variability in 
      gene copy number in tumors that had evidence of amplification; however, tumors 
      judged to be non-amplified completely lacked any cells with high N-myc copy 
      number. FISH provided reliable estimates of N-myc amplification in 12 clinical 
      samples even when the percentage of tumor was low. The other advantages of FISH 
      over Southern blot analysis were speed and technical simplicity, ability to 
      discern heterogeneous gene amplification among tumor cells in the same specimen, 
      and capacity to determine the source of the amplified N-myc signal, whether 
      extrachromosomal double-minute chromosomes, expanded intrachromosomal regions, or 
      chromosome 2 aneuploidy. We conclude that FISH would refine the analysis of N-myc 
      amplification in neuroblastoma and thus improve the assignment of patients to 
      prognostic groups based on this unfavorable risk factor.
FAU - Shapiro, D N
AU  - Shapiro DN
AD  - Department of Experimental Oncology, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Valentine, M B
AU  - Valentine MB
FAU - Rowe, S T
AU  - Rowe ST
FAU - Sinclair, A E
AU  - Sinclair AE
FAU - Sublett, J E
AU  - Sublett JE
FAU - Roberts, W M
AU  - Roberts WM
FAU - Look, A T
AU  - Look AT
LA  - eng
GR  - CA-21765/CA/NCI NIH HHS/United States
GR  - CA-23099/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
SB  - IM
MH  - Blotting, Southern
MH  - Child, Preschool
MH  - Chromosomes
MH  - Densitometry
MH  - *Gene Amplification
MH  - *Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Neuroblastoma/*diagnosis/genetics
MH  - Staining and Labeling
MH  - Tumor Cells, Cultured
PMC - PMC1886925
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
PMCR- 1993/11/01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
PHST- 1993/11/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1993 May;142(5):1339-46.