PMID- 7684485
OWN - NLM
STAT- MEDLINE
DCOM- 19930624
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 18
IP  - 3
DP  - 1993 May
TI  - Altered levels and splicing of the amyloid precursor protein in the adult rat 
      hippocampus after treatment with DMSO or retinoic acid.
PG  - 259-66
AB  - Alzheimer's disease and cognitive impairment in rats has been associated with an 
      increase in the percentage of amyloid precursor protein (APP) containing the KPI 
      domain. It has recently been reported that retinoic acid (RA) is capable of 
      increasing the levels and altering the splicing ratio of APP in cultured SH-SY5Y 
      cells. The effects of peripherally administered RA (64 or 640 micrograms/kg; 
      i.p.; q.d.) on the abundance of APP, the ratio of the three major isoforms, and 
      the relative abundance of nerve growth factor (NGF), brain derived neurotrophic 
      factor (BDNF), and neurotrophin-3 (NT-3) were determined by rtPCR in the 
      hippocampus of aged rats. Corresponding changes in choline acetyltransferase 
      (ChAT) activity were also measured. Vehicle (DMSO) treated rats exhibited a 2 x 
      (P < 0.01) increase in total APP and an 8 x (P < 0.001) decrease in the 
      cyclophilin transcript. In addition, DMSO increased the percentage of APP 695 
      from 89% in saline treated rats to 94%. Treatment of RA in DMSO decreased the 
      accumulation of total APP relative to cyclophilin at both the low (6.4 x; P < 
      0.01) and high (8 x; P < 0.05) dosages when compared to DMSO treated rats. 
      Furthermore, the level of APP-695 decreased to 82% with low dosage of RA and 75% 
      at high dosage of the total APP transcripts. No significant change in either NGF, 
      NT-3, or BDNF transcripts were observed following low or high dosage RA 
      administration relative to cyclophilin RNA nor was a change in ChAT activity 
      detected at either of the dosages tested.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Pan, J B
AU  - Pan JB
AD  - Abbott Laboratories, Abbott Park, IL 60064-3500.
FAU - Monteggia, L M
AU  - Monteggia LM
FAU - Giordano, T
AU  - Giordano T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cyclosporins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 5688UTC01R (Tretinoin)
RN  - 63231-63-0 (RNA)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 5.1.1.- (Amino Acid Isomerases)
RN  - EC 5.2.1.8 (Peptidylprolyl Isomerase)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Alternative Splicing/*drug effects
MH  - Amino Acid Isomerases/genetics/metabolism
MH  - Amyloid beta-Protein Precursor/*genetics/isolation & purification/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/genetics/metabolism
MH  - Choline O-Acetyltransferase/metabolism
MH  - Cyclosporins/metabolism
MH  - Dimethyl Sulfoxide/*pharmacology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Hippocampus/drug effects/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Oligonucleotides, Antisense
MH  - Peptidylprolyl Isomerase
MH  - Polymerase Chain Reaction/methods
MH  - RNA/genetics/isolation & purification
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - Tretinoin/*pharmacology
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 0169-328X(93)90198-X [pii]
AID - 10.1016/0169-328x(93)90198-x [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1993 May;18(3):259-66. doi: 
      10.1016/0169-328x(93)90198-x.