PMID- 7684959
OWN - NLM
STAT- MEDLINE
DCOM- 19930702
LR  - 20220310
IS  - 0009-921X (Print)
IS  - 0009-921X (Linking)
IP  - 291
DP  - 1993 Jun
TI  - The expression of major histocompatibility antigens on human articular 
      chondrocytes.
PG  - 266-82
AB  - Human articular chondrocytes were analyzed for major histocompatibility (MHC) 
      antigens. The cells were obtained by enzymatic digestion of surgical specimens 
      from patients with osteoarthrosis and rheumatoid arthritis having joint 
      replacement. The analyses of Class I and Class II antigens were performed using 
      microcytotoxicity techniques, as with conventional human leukocyte antigen (HLA) 
      typing, and by immunofluorescence on a flow cytometer with monoclonal antibodies. 
      Eighty-five percent of the chondrocytes had strong representation of Class I 
      molecules, and individual specificities for HLA-A, HLA-B, and HLA-C could be 
      detected. Class II antigens generally were not expressed on chondrocytes from 
      noninflammatory states. In osteoarthrosis, some cells expressed HLA-DP and 
      HLA-DQ, whereas in rheumatoid arthritis, there was an increase in the expression 
      of all Class II antigens. Incubation of chondrocytes with gamma-interferon caused 
      the strong induction of HLA-DR and HLA-DP, whereas HLA-DQ expression was largely 
      unaffected. These results have broad implications for tissue transplantation and 
      autoimmune disease.
FAU - Lance, E M
AU  - Lance EM
AD  - Shriners Hospitals for Crippled Children, Honolulu Unit, Hawaii 96826.
FAU - Kimura, L H
AU  - Kimura LH
FAU - Manibog, C N
AU  - Manibog CN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Orthop Relat Res
JT  - Clinical orthopaedics and related research
JID - 0075674
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cartilage, Articular/cytology/*immunology
MH  - Epitopes
MH  - Flow Cytometry
MH  - HLA Antigens/*analysis
MH  - HLA-A Antigens/analysis
MH  - HLA-D Antigens/analysis
MH  - Histocompatibility Antigens Class I/analysis
MH  - Histocompatibility Antigens Class II/analysis
MH  - Humans
MH  - T-Lymphocytes/immunology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - Clin Orthop Relat Res. 1993 Jun;(291):266-82.