PMID- 7684997
OWN - NLM
STAT- MEDLINE
DCOM- 19930707
LR  - 20190830
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 59
IP  - 1
DP  - 1993 Apr
TI  - Immunocytochemical study with anti-muscle actin antibody (HHF35) on myocardial 
      ischaemia and reperfusion injury in rats.
PG  - 25-34
AB  - Myocardial ischaemia and reperfusion injury in 16 anaesthetized Sprague-Dawley 
      rats, eight of which were pretreated with morphine (5 mg/kg, intraperitoneally) 
      to prevent arrhythmias, were studied immunocytochemically with anti-muscle actin 
      specific monoclonal antibody (HHF35). Eight cases of simple ischaemia and eight 
      cases of sham-operated rats were used as controls. With HHF35 ABC 
      immunocytochemical method, the left ventricular myocardium in the reperfusion 
      group (without morphine) showed large areas of staining loss, but no loss of 
      staining was seen in controls. No significant changes were seen with H&E stain in 
      all hearts. Compared with HBFP, acridine orange (AO), eosin-fluorescence, HHF35 
      ABC staining best demonstrated myocardial reperfusion injury. The results 
      indicate that the degree of myocardial damage may be related to the arrhythmias.
FAU - Fu, C
AU  - Fu C
AD  - Department of Forensic Pathology, Sun Yat-sen University of Medical Sciences, 
      Guangzhou, P.R. China.
FAU - Song, Y
AU  - Song Y
FAU - Zhu, J
AU  - Zhu J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Antibodies, Monoclonal)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Arrhythmias, Cardiac/complications/prevention & control
MH  - False Positive Reactions
MH  - Immunohistochemistry
MH  - Male
MH  - Morphine/therapeutic use
MH  - Myocardial Ischemia/etiology/*pathology
MH  - Myocardium/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*pathology
MH  - Staining and Labeling
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - 0379-0738(93)90309-X [pii]
AID - 10.1016/0379-0738(93)90309-x [doi]
PST - ppublish
SO  - Forensic Sci Int. 1993 Apr;59(1):25-34. doi: 10.1016/0379-0738(93)90309-x.