PMID- 7686167
OWN - NLM
STAT- MEDLINE
DCOM- 19930727
LR  - 20181130
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 156
IP  - 1
DP  - 1993 Jul
TI  - Pretreatment with vitamin A inhibits transforming growth factor alpha stimulation 
      of human mammary carcinoma cells.
PG  - 80-7
AB  - Vitamin A or retinol is an important agent in the normal differentiation and 
      growth of cells. Retinol is an effective inhibitor of the growth of many 
      transformed cells in vitro and in vivo but its mechanism of action is unclear. 
      Transforming growth factor alpha (TGF alpha) is a known mitogen. We examined the 
      effect of retinol treatment on TGF alpha stimulation of two human mammary 
      carcinoma cell lines, one which is growth inhibited by retinol and one which is 
      not. Pretreatment of both cell lines for 48 hours with retinol resulted in 
      inhibition of TGF alpha stimulation of growth. In the T47D cell line the 
      mechanism was not related to an effect on the cellular content of TGF alpha, 
      epidermal growth factor (EGF) receptor protein, EGF receptor mRNA, or on the 
      binding of TGF alpha to the EGF receptor. However, TGF alpha-induced stimulation 
      of the EGF receptor substrate, phospholipase C-gamma 1, was abrogated in the T47D 
      cell line with retinol pretreatment. In the MDA-MB-468 cell line, pretreatment 
      with retinol resulted in a decrease in tyrosine phosphorylation of the EGF 
      receptor. These results suggest that pretreatment with retinol decreases cellular 
      proliferation seen with TGF alpha treatment by altering phospholipase C-gamma 1 
      response and/or EGF receptor tyrosine kinase activity. Alteration of 
      phospholipase C-gamma 1 activity does not appear to be responsible for the 
      inhibition of cell growth seen in the absence of TGF alpha stimulation.
FAU - Halter, S A
AU  - Halter SA
AD  - Department of Pathology, Vanderbilt University, Nashville, Tennessee.
FAU - Winnier, A R
AU  - Winnier AR
FAU - Arteaga, C L
AU  - Arteaga CL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 11103-57-4 (Vitamin A)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Breast Neoplasms/*pathology
MH  - Cell Division/*drug effects
MH  - ErbB Receptors/metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Phosphotyrosine
MH  - RNA, Messenger/genetics
MH  - Signal Transduction/drug effects
MH  - Transforming Growth Factor alpha/*antagonists & inhibitors/metabolism
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/metabolism
MH  - Tyrosine/analogs & derivatives/metabolism
MH  - Vitamin A/*pharmacology
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1002/jcp.1041560112 [doi]
PST - ppublish
SO  - J Cell Physiol. 1993 Jul;156(1):80-7. doi: 10.1002/jcp.1041560112.