PMID- 7686254
OWN - NLM
STAT- MEDLINE
DCOM- 19930726
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 288
IP  - 1
DP  - 1993 Jul
TI  - Mutational analysis using denaturing gradient gel electrophoresis and PCR.
PG  - 103-12
AB  - Denaturing gradient gel electrophoresis (DGGE) separates (DNA) molecules based on 
      their sequence. Using the proper conditions, all base-pair substitutions can be 
      resolved from the wild-type sequence using DGGE. Polymerase chain reaction (PCR) 
      permits rapid amplification of a given region of the genome. In this paper, we 
      demonstrate the utility of DGGE combined with PCR for mutation analysis by 
      presenting different examples: (i) analysis of mouse p53 cDNA for mutations, (ii) 
      simultaneous analysis of thousands of 4NQO-induced mutants for mutations in HPRT 
      exon 3, (iii) examination of the fidelity of the thermostable DNA polymerase 
      isolated from Pyrococcus furiosus (Pfu), (iv) purification of mutant DNA from 
      contaminating wild-type DNA from mouse spleenic T-cell clones.
FAU - Cariello, N F
AU  - Cariello NF
AD  - University of North Carolina, Pathology Department, Chapel Hill 27599.
FAU - Skopek, T R
AU  - Skopek TR
LA  - eng
GR  - NRSA ES-05534-01/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 56-57-5 (4-Nitroquinoline-1-oxide)
RN  - 9007-49-2 (DNA)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - EC 2.7.7.- (Taq Polymerase)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - FTK8U1GZNX (Thioguanine)
SB  - IM
MH  - 4-Nitroquinoline-1-oxide/toxicity
MH  - Animals
MH  - Archaea/enzymology
MH  - Cells, Cultured
MH  - DNA/analysis/drug effects
MH  - DNA Mutational Analysis/*methods
MH  - DNA, Single-Stranded/*analysis
MH  - DNA-Directed DNA Polymerase
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - Genes, p53
MH  - Humans
MH  - Hypoxanthine Phosphoribosyltransferase/genetics
MH  - Mice
MH  - Mutagenesis
MH  - Nucleic Acid Conformation
MH  - Nucleic Acid Denaturation
MH  - Nucleic Acid Heteroduplexes/analysis
MH  - Polymerase Chain Reaction/*methods
MH  - T-Lymphocytes
MH  - Taq Polymerase
MH  - Thioguanine/pharmacology
RF  - 37
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 0027-5107(93)90212-X [pii]
AID - 10.1016/0027-5107(93)90212-x [doi]
PST - ppublish
SO  - Mutat Res. 1993 Jul;288(1):103-12. doi: 10.1016/0027-5107(93)90212-x.