PMID- 7687301
OWN - NLM
STAT- MEDLINE
DCOM- 19930816
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 8
DP  - 1993 Aug
TI  - Identification and mapping of the gene encoding the glycoprotein complex 
      gp82-gp105 of human herpesvirus 6 and mapping of the neutralizing epitope 
      recognized by monoclonal antibodies.
PG  - 4611-20
AB  - Monoclonal antibodies (MAbs) 2D4, 2D6, and 13D6 against human herpesvirus 6 
      (HHV-6) variant A strain GS recognized virion envelope glycoprotein complex 
      gp82-gp105 and neutralized the infectivity of HHV-6 variant A group isolates. A 
      624-bp genomic fragment (82G) was identified from an HHV-6 strain GS genomic 
      library constructed in the lambda gt11 expression system by immunoscreening with 
      MAb 2D6. Rabbit antibodies against the fusion protein expressed from the genomic 
      insert recognized glycoprotein complex gp82-gp105 from HHV-6-infected cells, thus 
      confirming that the genomic fragment is a portion of the gene(s) that encodes 
      gp82-gp105. This genomic insert hybridized specifically with viral DNAs from 
      HHV-6 variant A strains GS and U1101 under high-stringency conditions but 
      hybridized with HHV-6 variant B strain Z-29 DNA only under low-stringency 
      conditions. DNA sequence analysis of the insert revealed a 167-amino-acid single 
      open reading frame with an open 5' end and a stop codon at the 3' end. 
      Hybridization studies with HHV-6A strain U1102 DNA localized the 
      gp82-gp105-encoding gene to the unique long region near the direct repeat at the 
      right end of the genome. To locate the neutralizing epitope(s) recognized by the 
      MAbs, a series of deletions from the 3' end of the gene were constructed with 
      exonuclease III, and fusion proteins from deletion constructs were tested for 
      reactivity with MAbs in a Western immunoblot assay. Sequencing of deletion 
      constructs at the reactive-nonreactive transition point localized the epitope 
      recognized by the three neutralizing MAbs within or near a repeat amino acid 
      sequence (NIYFNIY) of the putative protein. This repeat sequence region is 
      surrounded on either side by two potential N-glycosylation sites and three 
      cysteine residues.
FAU - Pfeiffer, B
AU  - Pfeiffer B
AD  - Department of Microbiology, Molecular Genetics and Immunology, University of 
      Kansas Medical Center, Kansas City 66160.
FAU - Berneman, Z N
AU  - Berneman ZN
FAU - Neipel, F
AU  - Neipel F
FAU - Chang, C K
AU  - Chang CK
FAU - Tirwatnapong, S
AU  - Tirwatnapong S
FAU - Chandran, B
AU  - Chandran B
LA  - eng
SI  - GENBANK/L18985
GR  - AI24224/AI/NIAID NIH HHS/United States
GR  - AI30356/AI/NIAID NIH HHS/United States
GR  - AI33502/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, env)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Line
MH  - Chromosome Mapping
MH  - DNA, Viral/genetics/isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes/analysis
MH  - Gene Products, env/biosynthesis/*genetics/immunology
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - RNA, Viral/genetics/isolation & purification
MH  - Rabbits/immunology
MH  - Recombinant Fusion Proteins/biosynthesis/immunology/isolation & purification
MH  - Restriction Mapping
MH  - Sequence Deletion
MH  - T-Lymphocytes
PMC - PMC237846
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
PMCR- 1993/08/01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
PHST- 1993/08/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.8.4611-4620.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Aug;67(8):4611-20. doi: 10.1128/JVI.67.8.4611-4620.1993.