PMID- 7688741
OWN - NLM
STAT- MEDLINE
DCOM- 19930916
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 268
IP  - 24
DP  - 1993 Aug 25
TI  - A protein tyrosine phosphatase activity associated with the hepatocyte growth 
      factor/scatter factor receptor.
PG  - 18176-80
AB  - The receptor for the growth and motility factor, hepatocyte growth factor/scatter 
      factor (HGF/SF), is a transmembrane tyrosine kinase encoded by the MET oncogene. 
      Previous work has shown that receptor phosphorylation on tyrosine is critical for 
      both kinase activation and association with intracellular signal transducers. In 
      this paper, we report that a protein tyrosine phosphatase activity (PTP) 
      coprecipitates with the HGF/SF receptor. The associated PTP activity correlates 
      with the kinase activation of the receptor, increasing up to 5-fold over the 
      basal level after HGF/SF stimulation. The increase is reversible and time- and 
      dose-dependent. A comparable level of activity is associated with constitutively 
      tyrosine-phosphorylated receptors immunoprecipitated from cells where the MET 
      oncogene is amplified and overexpressed. In these cells, a parallel decrease in 
      PTP activity is observed after inhibition of receptor tyrosine phosphorylation 
      following protein kinase C activation. The associated PTP activity is effective 
      in dephosphorylating the HGF/SF receptor. These data show that a protein tyrosine 
      phosphatase is functionally coupled to the HGF/SF receptor.
FAU - Villa-Moruzzi, E
AU  - Villa-Moruzzi E
AD  - Department of Experimental Biomedicine, University of Pisa, Italy.
FAU - Lapi, S
AU  - Lapi S
FAU - Prat, M
AU  - Prat M
FAU - Gaudino, G
AU  - Gaudino G
FAU - Comoglio, P M
AU  - Comoglio PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Cell Line
MH  - Cell Membrane
MH  - Chromatography, Gel
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/*metabolism/pharmacology
MH  - Humans
MH  - Kinetics
MH  - Oncogenes
MH  - Phosphotyrosine
MH  - Protein Kinase C/metabolism
MH  - Protein Tyrosine Phosphatases/isolation & purification/*metabolism
MH  - Protein-Tyrosine Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins/genetics/isolation & purification/*metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Proto-Oncogenes
MH  - Recombinant Proteins/metabolism
MH  - Time Factors
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Tyrosine/analogs & derivatives/metabolism
EDAT- 1993/08/25 00:00
MHDA- 1993/08/25 00:01
CRDT- 1993/08/25 00:00
PHST- 1993/08/25 00:00 [pubmed]
PHST- 1993/08/25 00:01 [medline]
PHST- 1993/08/25 00:00 [entrez]
AID - S0021-9258(17)46826-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1993 Aug 25;268(24):18176-80.