PMID- 7689029
OWN - NLM
STAT- MEDLINE
DCOM- 19930917
LR  - 20211203
IS  - 0940-9912 (Print)
IS  - 0940-9912 (Linking)
VI  - 2
IP  - 2
DP  - 1993 Apr
TI  - Phenotypic modulation of human hair matrix cells (trichocytes) by environmental 
      influence in vitro and in vivo.
PG  - 55-65
AB  - Trichocytes, i.e. precursor cells of the hair cortex and medulla, isolated from 
      plucked human scalp hair follicles (HF) were propagated on feeder layers of 
      post-mitotic human dermal fibroblasts (HDF). Cell isolates from five HF routinely 
      yielded about 0.5-1 x 10(5) cells within 3 weeks. When grown as 'surface 
      epithelia' in vitro (on dermal equivalents exposed to air), trichocytes organized 
      into stratified epithelia largely reminiscent of epidermis with regard to both 
      tissue architecture and localization of epidermal differentiation products 
      (keratins K1 and K10, involucrin, filaggrin). However, when HDF in the collagen 
      matrix were replaced by dermal papilla cells (DPC) epidermoid differentiation was 
      largely prevented while still allowing growth and stratification. Epidermal 
      differentiation (keratinization) was virtually complete when trichocytes grown on 
      collagen gels with HDF were transplanted onto nude mice; this was apparent by 
      tissue organization, expression of K1 and K10 and the nearly regular epidermal 
      localization of involucrin. In addition, the deposition of basement membrane 
      components (laminin, type IV collagen, bullous pemphigoid antigen) at the 
      epithelium-collagen interface further increased and was more regular in 
      transplants than in vitro. Cells embedded in Matrigel together with HDF developed 
      large spheroidal structures with inward-directed differentiation and all the 
      epidermal markers found in 'surface' cultures, while only small keratinizing 
      spheroids formed without HDF. In this system co-culture of trichocytes with DPC 
      suppressed almost completely epidermal keratinization. Although typical hair 
      proteins were not detectable, our data clearly demonstrate that: (1) bona fide 
      trichocytes inherit the options for alternative directions of differentiation, 
      and (2) external (in part mesenchymal cell-mediated) influences play a pivotal 
      role in this determination.
FAU - Limat, A
AU  - Limat A
AD  - Cosmital SA, Marly, Switzerland.
FAU - Breitkreutz, D
AU  - Breitkreutz D
FAU - Thiekoetter, G
AU  - Thiekoetter G
FAU - Noser, F
AU  - Noser F
FAU - Hunziker, T
AU  - Hunziker T
FAU - Braathen, L R
AU  - Braathen LR
FAU - Fusenig, N E
AU  - Fusenig NE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Epithelial Cell Biol
JT  - Epithelial cell biology
JID - 9206038
RN  - 0 (Drug Combinations)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 68238-35-7 (Keratins)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Cells, Cultured
MH  - Collagen
MH  - Drug Combinations
MH  - Epidermal Cells
MH  - Fibroblasts/cytology
MH  - Filaggrin Proteins
MH  - Fluorescent Antibody Technique
MH  - Hair/chemistry/*cytology/transplantation
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratins/analysis
MH  - Laminin
MH  - Mice
MH  - Mice, Nude
MH  - Mitosis
MH  - Phenotype
MH  - Proteoglycans
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PST - ppublish
SO  - Epithelial Cell Biol. 1993 Apr;2(2):55-65.