PMID- 7690969
OWN - NLM
STAT- MEDLINE
DCOM- 19931020
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 18
DP  - 1993 Sep 15
TI  - Release from quiescence of CD34+ CD38- human umbilical cord blood cells reveals 
      their potentiality to engraft adults.
PG  - 8707-11
AB  - Using optimal culture conditions in which the transforming growth factor beta 1 
      (TGF-beta 1) inhibitory loop has been interrupted by antisense TGF-beta 1 
      oligonucleotides or anti-TGF-beta serum, we have compared the proliferative 
      capacities and the abilities of the CD34+ CD38- cell populations from bone marrow 
      and umbilical cord blood to generate early progenitors in long-term cultures. The 
      CD34+ CD38- fraction of umbilical cord blood accounts for 4% of the CD34+ 
      fraction compared to only 1% in bone marrow, indicating that umbilical cord blood 
      may be relatively enriched in stem cells. We estimate that the CD34+ CD38- cells 
      from a typical umbilical cord blood sample produce equivalent numbers of 
      colony-forming units (CFU)-granulocyte/erythrocyte/macrophage/megakaryocyte, 
      twice as many CFU-granulocyte/macrophage (GM) and 3 times as many burst-forming 
      units-erythroid as the same population from an average bone marrow sample used in 
      adult transplantation. In addition, the colonies resulting from the umbilical 
      cord blood samples were significantly larger than those from bone marrow, 
      indicating a greater growth potential. However, the content of later progenitors, 
      which may be important for short-term reconstitution, was less in umbilical cord 
      blood-derived than in bone marrow-derived cell preparations, as estimated by a 
      4-fold lower production of CFU-GM in long-term cultures of CD34+ CD38+ cells. 
      This deficit is partially compensated by the higher growth capacity of the 
      resulting CFU-GM. These studies suggest that umbilical cord blood is a suitable 
      source of cells for adult transplantation.
FAU - Cardoso, A A
AU  - Cardoso AA
AD  - Centre National de la Recherche Scientifique UPR 272, Villejuif, France.
FAU - Li, M L
AU  - Li ML
FAU - Batard, P
AU  - Batard P
FAU - Hatzfeld, A
AU  - Hatzfeld A
FAU - Brown, E L
AU  - Brown EL
FAU - Levesque, J P
AU  - Levesque JP
FAU - Sookdeo, H
AU  - Sookdeo H
FAU - Panterne, B
AU  - Panterne B
FAU - Sansilvestri, P
AU  - Sansilvestri P
FAU - Clark, S C
AU  - Clark SC
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Growth Substances)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.2.2.5 (ADP-ribosyl Cyclase)
RN  - EC 3.2.2.5 (CD38 protein, human)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase
MH  - ADP-ribosyl Cyclase 1
MH  - Adult
MH  - Antigens, CD/*blood
MH  - Antigens, CD34
MH  - Antigens, Differentiation/*blood
MH  - *Bone Marrow Cells
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Fetal Blood/*cytology/immunology
MH  - Growth Substances/*pharmacology
MH  - Hematopoietic Stem Cells/*cytology/drug effects/immunology
MH  - Humans
MH  - Kinetics
MH  - Membrane Glycoproteins
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Transforming Growth Factor beta/genetics/physiology
PMC - PMC47427
EDAT- 1993/09/15 00:00
MHDA- 1993/09/15 00:01
PMCR- 1994/03/15
CRDT- 1993/09/15 00:00
PHST- 1993/09/15 00:00 [pubmed]
PHST- 1993/09/15 00:01 [medline]
PHST- 1993/09/15 00:00 [entrez]
PHST- 1994/03/15 00:00 [pmc-release]
AID - 10.1073/pnas.90.18.8707 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8707-11. doi: 
      10.1073/pnas.90.18.8707.