PMID- 7691110
OWN - NLM
STAT- MEDLINE
DCOM- 19931105
LR  - 20131121
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 9
IP  - 4
DP  - 1993 Oct
TI  - Release of interleukin-8, interleukin-6, and colony-stimulating factors by upper 
      airway epithelial cells: implications for cystic fibrosis.
PG  - 455-62
AB  - Cystic fibrosis (CF) is characterized by a dramatic neutrophil recruitment and 
      repeated Pseudomonas infections in the lungs. To evaluate cytokine releasibility 
      by airway epithelial cells in the context of CF, we studied primary nasal 
      epithelial cells isolated from the upper airways and continuous epithelial cell 
      lines from normal and CF subjects. Relatively low levels of interleukin (IL)-8, 
      IL-6, and granulocyte/macrophage colony-stimulating factor (GM-CSF) were produced 
      spontaneously by primary epithelial cells (< 50 pg/10(6) cells) and higher levels 
      of colony-stimulating factor-1 (CSF-1) (1 to 2 ng/10(6) cells). Cells were 
      stimulated with substances that are likely to be present in the inflamed lungs of 
      CF patients-namely, the proinflammatory monokines IL-1 and tumor necrosis 
      factor-alpha (TNF alpha) as well as neutrophil elastase and bacterial products 
      from Pseudomonas (mucoid exopolysaccharide [MEP] and rhamnolipids). Both IL-1 and 
      TNF alpha induced a dose-dependent release of IL-6 (5 to 10 ng/10(6) cells) and 
      GM-CSF (2 to 3 ng/10(6) cells) by primary epithelial cells from eight normal 
      volunteers. The TNF alpha/IL-1-stimulated GM-CSF release was blocked by the 
      addition of 1 microM dexamethasone, whereas basal CSF-1 release was unaffected. 
      Neutrophil elastase was a potent inducer of IL-8 and GM-CSF both in primary 
      epithelial cells and in cell lines. Dexamethasone (1 microM) did not inhibit 
      elastase-induced IL-8 release in either normal or CF epithelial cells. 
      Rhamnolipids and MEP were found to stimulate the copious release of IL-8, GM-CSF, 
      and IL-6 from epithelial cells, in a steroid-sensitive fashion.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Bedard, M
AU  - Bedard M
AD  - Department of Anatomy and Cellular Biology, Faculty of Medicine, University of 
      Sherbrooke, Quebec, Canada.
FAU - McClure, C D
AU  - McClure CD
FAU - Schiller, N L
AU  - Schiller NL
FAU - Francoeur, C
AU  - Francoeur C
FAU - Cantin, A
AU  - Cantin A
FAU - Denis, M
AU  - Denis M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (CFTR protein, human)
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Membrane Proteins)
RN  - 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Analysis of Variance
MH  - Cells, Cultured
MH  - Colony-Stimulating Factors/*metabolism
MH  - Cystic Fibrosis/immunology/*metabolism
MH  - Cystic Fibrosis Transmembrane Conductance Regulator
MH  - Dexamethasone/pharmacology
MH  - Epithelial Cells
MH  - Epithelium/drug effects/metabolism
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Interleukin-8/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Nasal Mucosa/cytology/metabolism
MH  - Phenotype
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 10.1165/ajrcmb/9.4.455 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1993 Oct;9(4):455-62. doi: 10.1165/ajrcmb/9.4.455.