PMID- 7691162
OWN - NLM
STAT- MEDLINE
DCOM- 19931119
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 8
IP  - 1
DP  - 1993 Sep
TI  - Chromosomal radiosensitivity at intrachromosomal telomeric sites.
PG  - 8-14
AB  - Telomeric DNA is composed of highly conserved sequences which are present at the 
      termini of chromosomes as well as at intrachromosomal locations. Here, we studied 
      a Chinese hamster ovary (CHO) cell line, BL-10, with highly stable amplified 
      telomeric DNA at the termini as well as at intrachromosomal locations. We show 
      that intrachromosomal or interstitial telomeric sites in this cell line and in 
      another CHO cell line, HA-I, are radiosensitive in that they are more prone to 
      breakage than would be expected based on the percentage of the genome composed of 
      telomeric sequences. The frequency of breakage at interstitial telomeric sites is 
      4.3 to 8.3 times higher than that in the CHO genome overall. These conclusions 
      are reached by both conventional cytogenetic analysis of two CHO cell lines which 
      have the same survival rates after exposure to ionizing radiation, and by use of 
      double fluorescence in situ hybridization (FISH) with a pan-telomere-specific 
      probe and a CHO chromosome-specific library in the same metaphase cells after 
      irradiation.
FAU - Alvarez, L
AU  - Alvarez L
AD  - Stanford University School of Medicine, Cancer Biology Research Laboratory, CA 
      94305-5468.
FAU - Evans, J W
AU  - Evans JW
FAU - Wilks, R
AU  - Wilks R
FAU - Lucas, J N
AU  - Lucas JN
FAU - Brown, J M
AU  - Brown JM
FAU - Giaccia, A J
AU  - Giaccia AJ
LA  - eng
GR  - CA 55801/CA/NCI NIH HHS/United States
GR  - CA 58838/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Survival/radiation effects
MH  - *Chromosome Aberrations
MH  - Chromosomes/*radiation effects
MH  - Clone Cells
MH  - Cricetinae
MH  - DNA/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Flow Cytometry
MH  - Gene Amplification
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - Radiation, Ionizing
MH  - Telomere/*radiation effects
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1002/gcc.2870080103 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1993 Sep;8(1):8-14. doi: 10.1002/gcc.2870080103.