PMID- 7691200
OWN - NLM
STAT- MEDLINE
DCOM- 19931026
LR  - 20180215
IS  - 1018-1172 (Print)
IS  - 1018-1172 (Linking)
VI  - 30
IP  - 5
DP  - 1993 Sep-Oct
TI  - Structural heterogeneity of the diffuse intimal thickening and correlation with 
      distribution of TGF-beta 1.
PG  - 293-302
AB  - This study examines the ultrastructural features of diffuse intimal thickenings 
      of human coronary arteries and correlates structural heterogeneity along the 
      radial axis with the distribution and known actions of transforming growth factor 
      beta 1 (TGF-beta 1). Morphometric and immunohistochemical data were collected 
      from thickenings of varying widths, sampled at autopsy from 19 persons ranging in 
      age from 3 months to 81 years. Thickenings were characterised by an inner 
      proteoglycan layer (PGL), up to approximately 70 mm wide, and an underlying 
      variable-width musculofibrous layer (MFL). The PGL was characterised by low 
      volume fractions (v/fs) for collagen, elastin, basement membranes and cells and a 
      high v/f for matrix space; v/fs for the MFL components were more evenly 
      distributed. Proteoglycans were visualised by ruthenium red staining, quantified 
      and sized. Densities of large (versican; > 20 nm) and small (< 20 nm) granules 
      changed little across intimal thickenings. Mean diameters of matrix space 
      granules increased with increasing intimal thickness and notably were 
      significantly (p < 0.001) larger in the PGL than the MFL. In contrast, diameters 
      of collagen-associated small granules (decorin) did not differ between the PGL 
      and MFL. TGF-beta 1 staining was detected in 70% of vessels examined and occurred 
      almost exclusively in the PGL, although showed a patchy distribution. Both the 
      distribution of TGF-beta 1 and its known differential effects on versican and 
      decorin synthesis suggest that it may play a significant role in the formation 
      and maintenance of the PGL.
FAU - Merrilees, M J
AU  - Merrilees MJ
AD  - Department of Anatomy, School of Medicine, University of Auckland, New Zealand.
FAU - Beaumont, B
AU  - Beaumont B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (Proteoglycans)
RN  - 0 (Transforming Growth Factor beta)
RN  - 11103-72-3 (Ruthenium Red)
RN  - 9007-34-5 (Collagen)
RN  - 9007-58-3 (Elastin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arteries/anatomy & histology/cytology/metabolism
MH  - Child
MH  - Child, Preschool
MH  - Collagen/metabolism
MH  - Coronary Vessels/*anatomy & histology/cytology/*metabolism
MH  - Elastin/metabolism
MH  - Extracellular Matrix/ultrastructure
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Proteoglycans/metabolism
MH  - Ruthenium Red
MH  - Tissue Distribution
MH  - Transforming Growth Factor beta/*metabolism
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1159/000159008 [doi]
PST - ppublish
SO  - J Vasc Res. 1993 Sep-Oct;30(5):293-302. doi: 10.1159/000159008.