PMID- 7691479 OWN - NLM STAT- MEDLINE DCOM- 19931122 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 65 IP - 1-2 DP - 1994 TI - Human alpha-satellite DNAs are not susceptible to undercondensation after 5-azacytidine treatment. PG - 92-4 AB - Localization of alphoid human satellite DNAs using fluorescence in situ hybridization (FISH) of metaphase chromosomes following treatment with 5-azacytidine to produce undercondensation showed that human alpha-satellite DNA is not sensitive to the condensation-inhibition effect of 5-azacytidine. The difference in heterochromatic DNA subsets was particularly evident in the constitutive heterochromatin of chromosomes 1 and 9. Comparison of the results obtained after FISH with those obtained from electron microscopy and G-banding enabled accurate localization of this DNA domain. FAU - Fernandez, J L AU - Fernandez JL AD - Centro Oncologico de Galicia, La Coruna, Spain. FAU - Goyanes, V AU - Goyanes V FAU - Lopez-Fernandez, C AU - Lopez-Fernandez C FAU - Gosalvez, J AU - Gosalvez J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (DNA, Satellite) RN - M801H13NRU (Azacitidine) SB - IM MH - Azacitidine/*pharmacology MH - Chromosome Banding MH - DNA, Satellite/analysis/*drug effects/ultrastructure MH - Humans MH - In Situ Hybridization, Fluorescence EDAT- 1994/01/01 00:00 MHDA- 1994/01/01 00:01 CRDT- 1994/01/01 00:00 PHST- 1994/01/01 00:00 [pubmed] PHST- 1994/01/01 00:01 [medline] PHST- 1994/01/01 00:00 [entrez] AID - 10.1159/000133607 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1994;65(1-2):92-4. doi: 10.1159/000133607.