PMID- 7691636
OWN - NLM
STAT- MEDLINE
DCOM- 19931101
LR  - 20220321
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 21
IP  - 11
DP  - 1993 Oct
TI  - Differential regulation of the expression of CD38 and human leukocyte antigen-DR 
      on CD34+ hematopoietic progenitor cells by interleukin-4 and interferon-gamma.
PG  - 1480-6
AB  - We studied the effects of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) 
      on the expression of CD38 and human leukocyte antigen (HLA)-DR on purified CD34+ 
      bone marrow progenitor cells. CD34+CD38- and CD34+HLA-DR- cells are largely 
      nonoverlapping populations. After culture for 4 days in IFN-gamma, the expression 
      of CD38 and HLA-DR is significantly increased and the disappearance of the CD38- 
      and HLA-DR- populations is virtually complete. Moreover, IFN-gamma induces a 
      population of CD34+ cells with a very high expression of CD38 (CD34+CD38++ 
      cells), which were absent in the initial CD34+ population. IL-4 has no effect on 
      the expression of CD38, but induces a limited but significant increase in the 
      expression of HLA-Dr. After culture in IFN-gamma, CD34+ cells show a higher 
      cloning efficiency of the colony-forming unit-macrophage (CFU-M) and 
      burst-forming unit-erythroid (BFU-E) compared to cells cultured in medium alone. 
      After culture in IL-4, a limited increase in CFU-granulocyte (CFU-G) and BFU-E is 
      seen, whereas CFU-G, CFU-M, and BFU-E are increased after culture in IL-4 plus 
      IFN-gamma. We further investigated the functional properties of the CD34+CD38++ 
      cells generated in the presence of IFN-gamma. This cell population is highly 
      enriched for BFU-E but partially depleted of CFU-M. Most of the CFU-M were found 
      in the CD34+CD38+/-(CD34+CD38- and CD34+CD38+ cells) population.
FAU - Snoeck, H W
AU  - Snoeck HW
AD  - Laboratory of Experimental Hematology, University of Antwerp (UIA), Belgium.
FAU - Lardon, F
AU  - Lardon F
FAU - Lenjou, M
AU  - Lenjou M
FAU - Nys, G
AU  - Nys G
FAU - Van Bockstaele, D R
AU  - Van Bockstaele DR
FAU - Peetermans, M E
AU  - Peetermans ME
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.2.2.5 (ADP-ribosyl Cyclase)
RN  - EC 3.2.2.5 (CD38 protein, human)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase
MH  - ADP-ribosyl Cyclase 1
MH  - Antigens, CD/*analysis
MH  - Antigens, CD34
MH  - Antigens, Differentiation/*biosynthesis
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Colony-Forming Units Assay
MH  - HLA-DR Antigens/*biosynthesis
MH  - Hematopoietic Stem Cells/cytology/drug effects/*immunology
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Membrane Glycoproteins
MH  - Recombinant Proteins/pharmacology
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1993 Oct;21(11):1480-6.