PMID- 7692974
OWN - NLM
STAT- MEDLINE
DCOM- 19931202
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1216
IP  - 1
DP  - 1993 Oct 19
TI  - Pausing by retroviral DNA polymerases promotes strand transfer from internal 
      regions of RNA donor templates to homopolymeric acceptor templates.
PG  - 20-30
AB  - We have examined the ability of reverse transcriptases (RT) to catalyze strand 
      transfer from internal regions of RNA templates, resulting in switching of a 
      primer from one template to another. To study this phenomenon, we employed a 
      system of donor and acceptor templates in which homologous strand transfer can 
      occur from a homopolymeric sequence, positioned internally on the donor template. 
      Our results indicate that reverse transcriptases from human immunodeficiency 
      virus (HIV), avian myeloblastosis virus (AMV), and murine leukemia virus (MuLV) 
      are all able to catalyze strand transfer from this sequence. Catalysis of this 
      reaction is not dependent upon ribonuclease H (RNase H) activity, since an RNase 
      H-deficient form of HIV-RT is able to catalyze the reaction efficiently. 
      Additionally, N-ethylmaleimide, which inhibits RNase H but not polymerase 
      activity, did not inhibit the template switching by either the native or RNase 
      H-deficient forms of HIV-RT. Our data further indicate that template switching 
      may be promoted by RT pausing at a specific site on the donor template. 
      Conditions that increase RT pausing at this site also increase template 
      switching. These results suggest that transient RT pausing at specific sites on 
      the viral genome during reverse transcription may promote template switches that 
      in turn lead to recombination.
FAU - Buiser, R G
AU  - Buiser RG
AD  - Department of Biochemistry, University of Rochester, NY 14642.
FAU - Bambara, R A
AU  - Bambara RA
FAU - Fay, P J
AU  - Fay PJ
LA  - eng
GR  - AI 27054/AI/NIAID NIH HHS/United States
GR  - CA 11193/CA/NCI NIH HHS/United States
GR  - GM 49573/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - EC 3.1.26.4 (Ribonuclease H)
SB  - IM
MH  - DNA-Directed DNA Polymerase/*genetics
MH  - *RNA, Viral
MH  - RNA-Directed DNA Polymerase/metabolism
MH  - Retroviridae/*genetics
MH  - Ribonuclease H
MH  - *Templates, Genetic
EDAT- 1993/10/19 00:00
MHDA- 1993/10/19 00:01
CRDT- 1993/10/19 00:00
PHST- 1993/10/19 00:00 [pubmed]
PHST- 1993/10/19 00:01 [medline]
PHST- 1993/10/19 00:00 [entrez]
AID - 0167-4781(93)90033-A [pii]
AID - 10.1016/0167-4781(93)90033-a [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1993 Oct 19;1216(1):20-30. doi: 
      10.1016/0167-4781(93)90033-a.