PMID- 7693119
OWN - NLM
STAT- MEDLINE
DCOM- 19931222
LR  - 20191023
IS  - 0248-4900 (Print)
IS  - 0248-4900 (Linking)
VI  - 78
IP  - 1-2
DP  - 1993
TI  - Flow cytometry and RNA studies.
PG  - 27-30
AB  - RNA was less studied by flow cytometry (FCM) in comparison to other cellular 
      components. Moreover, most of these studies were applied to the measurement of 
      total RNA. Nevertheless, such a quantification, essentially by the use of 
      acridine orange, but also other dyes or dye combinations, permitted the 
      acquisition of important information about the general metabolism, and the 
      characterization of different tumoral cell types. More recently, FCM has been 
      used to quantify specific mRNA, in two different approaches: the first one 
      employs the sorting followed by RNA extraction and/or detection by specific 
      radiolabeled probes; in the second approach, the use of fluorescence in situ 
      hybridization (FISH) on cells in suspension allowed the direct quantification of 
      specific mRNA by FCM.
FAU - Grunwald, D
AU  - Grunwald D
AD  - DBMS/BMCC, INSERM U309, Grenoble, France.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Biol Cell
JT  - Biology of the cell
JID - 8108529
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 63231-63-0 (RNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - *Flow Cytometry
MH  - Fluorescent Dyes
MH  - Gene Expression
MH  - Humans
MH  - RNA/*analysis
MH  - RNA, Messenger/analysis
MH  - RNA, Neoplasm/analysis
RF  - 33
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 0248-4900(93)90111-Q [pii]
AID - 10.1016/0248-4900(93)90111-q [doi]
PST - ppublish
SO  - Biol Cell. 1993;78(1-2):27-30. doi: 10.1016/0248-4900(93)90111-q.