PMID- 7696569
OWN - NLM
STAT- MEDLINE
DCOM- 19950504
LR  - 20131121
IS  - 0006-3525 (Print)
IS  - 0006-3525 (Linking)
VI  - 35
IP  - 2
DP  - 1995 Feb
TI  - Fluorescence energy transfer in one dimension: frequency-domain fluorescence 
      study of DNA-fluorophore complexes.
PG  - 245-55
AB  - Fluorescence resonance energy transfer among linear DNA bound fluorophores was 
      carried out to study the process in one dimension. The donor fluorescence 
      intensity decays in the case of energy transfer in one dimension are stretched 
      exponential and show exp[-(t/tau)1/6] time dependence, which results in an 
      initial more rapid decay and subsequent slower decay at long times when compared 
      to those in higher dimensions. DNA-bound 4',6'-diamidino-2-phenyl indole (DAPI), 
      acridine orange (AO), and ethidium bromide (EB) were used as donors. The 
      acceptors were in the case of DAPI AO and EB; in the case of AO nile blue (NB), 
      methylene blue (MB), and crystal violet (CV); and NB, MB, and oxazine 750 in the 
      case of EB. As expected, the donor intensity decays became highly heterogeneous 
      upon energy transfer and were characterized by the simultaneous presence of both 
      highly and marginally quenched donors. The intensity decays for all three donors 
      in the presence of various acceptors are satisfactorily described by the Forster 
      model of energy transfer in one dimension. The intensity decays also allow for 
      clear rejection of a two- or three-dimensional model. The experimentally 
      recovered critical Forster distances (R0) ranged between 37 A in the case of DAPI 
      and EB to 70 A in the case of AO and CV donor-acceptor pairs. These recovered R0 
      values compare reasonably with those calculated from spectral properties if we 
      use values of 1.25 for k2, and 1.5 for the refractive index of DNA. The k2 value 
      will be even higher, between 1.5 and 2.0, if the consensus DNA refractive index 
      of 1.75 is used. These k2 values strongly suggest that the dipoles of the 
      acceptor chromophores when bound to DNA are not randomly oriented but are aligned 
      preferentially in plane.
FAU - Maliwal, B P
AU  - Maliwal BP
AD  - Department of Biological Chemistry, University of Maryland School of Medicine, 
      Baltimore 21201, USA.
FAU - Kusba, J
AU  - Kusba J
FAU - Lakowicz, J R
AU  - Lakowicz JR
LA  - eng
GR  - GM-35154/GM/NIGMS NIH HHS/United States
GR  - RR 07510/RR/NCRR NIH HHS/United States
GR  - TW-00049/TW/FIC NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biopolymers
JT  - Biopolymers
JID - 0372525
RN  - 0 (Indicators and Reagents)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Animals
MH  - Cattle
MH  - DNA/*chemistry
MH  - Energy Transfer
MH  - Ethidium/chemistry
MH  - Indicators and Reagents
MH  - Indoles/chemistry
MH  - Spectrometry, Fluorescence
MH  - Thymus Gland/chemistry
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1002/bip.360350213 [doi]
PST - ppublish
SO  - Biopolymers. 1995 Feb;35(2):245-55. doi: 10.1002/bip.360350213.