PMID- 7698011
OWN - NLM
STAT- MEDLINE
DCOM- 19950504
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 69
IP  - 3-4
DP  - 1995
TI  - Human cDNA mapping using a high-resolution R-banding technique and fluorescence 
      in situ hybridization.
PG  - 196-200
AB  - High-resolution fluorescence in situ hybridization (FISH) is now an essential 
      element in both human gene mapping and clinical cytogenetics. To facilitate its 
      application, a series of techniques have been developed using FISH to map DNA 
      probes in the size range of 1-1,000 kb directly on R-banded human chromosomes. 
      Distinctive reverse (R) banding is achieved by staining with chromomycin A3 and 
      distamycin A following in situ hybridization. The use of such counterstains 
      enables simultaneous viewing of both the fluorescent R-bands and in situ 
      hybridization signals by either standard photomicroscopy or an automated 
      image-acquisition system. This method is rapid and reproducibly reveals bands at 
      the 350-700 stage. Further, specific methods for chromosome preparation, 
      hybridization, and signal production have been developed and applied in 
      combination with R-banding. These methods are used for precise chromosomal 
      localization of DNA sequences in sizes ranging from that of cDNA (> 1 kb) through 
      bacterial artificial chromosomes (100-150 kb) to yeast artificial chromosomes (> 
      or = 1 Mb). These techniques provide high-resolution methods for rapid mapping of 
      human genes, expanding the applications of FISH techniques in basic research and 
      clinical analysis.
FAU - Korenberg, J R
AU  - Korenberg JR
AD  - Ahmanson Department of Pediatrics, Cedars-Sinai Medical Center, University of 
      California, Los Angeles 90048, USA.
FAU - Chen, X N
AU  - Chen XN
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA, Complementary)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Chromosome Banding
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Cosmids
MH  - DNA/*analysis
MH  - DNA, Complementary
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Plasmids
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000133962 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;69(3-4):196-200. doi: 10.1159/000133962.