PMID- 7698233
OWN - NLM
STAT- MEDLINE
DCOM- 19950428
LR  - 20090929
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 217
IP  - 2
DP  - 1995 Apr
TI  - The expression and release of adhesion molecules by human endothelial cell lines 
      and their consequent binding of lymphocytes.
PG  - 329-35
AB  - We report the characterization of a novel series of human endothelial cell lines 
      (designated SGHEC) regarding the expression and release of adhesion molecules and 
      their binding of lymphocytes. SGHEC expressed significant levels of intercellular 
      adhesion molecule-1 (ICAM-1; CD54) which increased after stimulation with tumor 
      necrosis factor-alpha (TNF alpha), interleukin-1 beta (IL-1 beta), or 
      interferon-gamma (IFN-gamma). Vascular cell adhesion molecule-1 (VCAM-1; CD106) 
      and E-selectin (CD62E) were not detectable on unstimulated SGHEC but substantial 
      levels were expressed after stimulation with either TNF alpha or IL-1 beta but 
      not with IFN-gamma. The increased expression of ICAM-1 and VCAM-1 was evident 
      after 4 h stimulation and was even higher after 24 h; E-selectin was maximal 
      after 4 h and returned almost to basal levels by 24 h. Substantial quantities of 
      immunoreactive ICAM-1 and VCAM-1 also accumulated as soluble material in the 
      supernatants of TNF alpha-stimulated SGHEC (VCAM-1 was substantially higher than 
      ICAM-1), but E-selectin remained below the limits of detection. Various 
      quantitative data suggest that this is a controlled release regulated by cytokine 
      and provide support for a physiological function for these soluble molecules. 
      Primary human lymphocytes and lymphoblastoid cell lines expressing lymphocyte 
      function-associated antigen-1 (LFA-1) bound to SGHEC; this binding increased 
      substantially after activation of either cell type. The binding was inhibited by 
      monoclonal antibodies against LFA-1 and, to a lesser extent, ICAM-1, thus 
      demonstrating the importance of these molecules in the observed binding; neither 
      anti-VCAM-1 nor anti-E-selectin antibodies affected the binding. From these 
      various data, we conclude that LFA-1/ICAM-1 interactions are partially 
      responsible for the binding of lymphocytes to endothelial cells. The SGHEC lines 
      should prove useful in investigating leukocyte-endothelial interactions and the 
      mechanism of release of soluble adhesion molecules.
FAU - Cartwright, J E
AU  - Cartwright JE
AD  - Department of Cellular and Molecular Sciences, St. George's Hospital Medical 
      School, London, United Kingdom.
FAU - Whitley, G S
AU  - Whitley GS
FAU - Johnstone, A P
AU  - Johnstone AP
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Line/*metabolism
MH  - Cytokines/physiology
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Lymphocytes/*metabolism
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - S0014-4827(85)71094-4 [pii]
AID - 10.1006/excr.1995.1094 [doi]
PST - ppublish
SO  - Exp Cell Res. 1995 Apr;217(2):329-35. doi: 10.1006/excr.1995.1094.