PMID- 7700634
OWN - NLM
STAT- MEDLINE
DCOM- 19950503
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 10
IP  - 6
DP  - 1995 Mar 16
TI  - A reciprocal translocation (1;15) (36.2;q24) in a neuroblastoma cell line is 
      accompanied by DNA duplication and may signal the site of a putative tumor 
      suppressor-gene.
PG  - 1095-101
AB  - Cytogenetic analyses and molecular deletion studies of human neuroblastomas have 
      indicated the chromosomal bands 1p36.1-1p36.2 as a location of genetic 
      information which may be involved in tumorigenesis. To define this putative 
      neuroblastoma locus in more detail we have analysed cell lines with alterations 
      of distal 1p. Here we show, by fluorescence in situ hybridization (FISH), that 
      cell line NGP has a reciprocal 1;15 translocation. Loci D1S214/D1S96 could be 
      shown to map telomeric/distal, D1S228 centromeric/proximal to the break. We have 
      identified yeast artificial chromosomes (YACs) that cover the break and map to 
      D1S160 and D1S244. This chromosomal position is within the smallest region of 
      overlap (SRO) found in neuroblastoma tumors (Weith et al., 1989; Caron et al., 
      1993; Schleiermacher et al., 1994) and within the region of a constitutional 
      interstitial deletion of a neuroblastoma patient (Biegel et al., 1993). Mapping 
      studies with FISH revealed that the translocation is associated with duplication 
      of DNA. It appears, as if the subchromosomal region we describe here is a good 
      candidate for harboring the postulated neuroblastoma suppressor-gene.
FAU - Amler, L C
AU  - Amler LC
AD  - Department of Cytogenetics, German Cancer Research Center, Heidelberg.
FAU - Corvi, R
AU  - Corvi R
FAU - Praml, C
AU  - Praml C
FAU - Savelyeva, L
AU  - Savelyeva L
FAU - Le Paslier, D
AU  - Le Paslier D
FAU - Schwab, M
AU  - Schwab M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
SB  - IM
EIN - Oncogene 1995 Dec 7;11(11):2453
MH  - Base Sequence
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 15
MH  - DNA Primers
MH  - DNA, Neoplasm/*genetics
MH  - *Genes, Retinoblastoma
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/03/16 00:00
MHDA- 1995/03/16 00:01
CRDT- 1995/03/16 00:00
PHST- 1995/03/16 00:00 [pubmed]
PHST- 1995/03/16 00:01 [medline]
PHST- 1995/03/16 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1995 Mar 16;10(6):1095-101.