PMID- 7703435
OWN - NLM
STAT- MEDLINE
DCOM- 19950509
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 6
IP  - 1
DP  - 1994 Dec 30
TI  - PAF antagonist, BN52021, inhibits [3H]D-aspartate release after ischaemia in 
      vitro.
PG  - 85-8
AB  - The effect of the platelet activating factor (PAF) antagonist BN52021 on 
      [3H]D-aspartate (D-Asp) release was investigated in rat hippocampal slices during 
      and after incubation (20 min) in ischaemia-like conditions. Ischaemia did not 
      influence spontaneous D-Asp outflow whereas K(+)-evoked, calcium-dependent 
      release was markedly enhanced in reoxygenated, post-ischaemic slices. These 
      slices also showed a substantial translocation/activation of protein kinase C 
      (PKC). BN52021 blocked both ischaemia-induced effects. Moreover, the PKC 
      inhibitor H7 attenuated post-ischaemic K(+)-evoked D-Asp release when beta-PDBu, 
      a PKC activator, was used to enhance the response of normoxic slices. Assuming 
      that PKC is activated by ischaemia in a PAF-dependent manner and that this 
      activation proceeds to enhanced glutamate exocytosis, we speculate on the 
      involvement of PAF receptor stimulation in the pathology of cerebral ischaemia.
FAU - Zaboocka, B
AU  - Zaboocka B
AD  - Department of Neurochemistry, Polish Academy of Sciences, Warsaw.
FAU - Domanska-Janik, K
AU  - Domanska-Janik K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Diterpenes)
RN  - 0 (Ginkgolides)
RN  - 0 (Isoquinolines)
RN  - 0 (Lactones)
RN  - 0 (Piperazines)
RN  - 0 (Platelet Activating Factor)
RN  - 10028-17-8 (Tritium)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - DF149B9460 (ginkgolide B)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - Animals
MH  - Aspartic Acid/*antagonists & inhibitors
MH  - Brain Ischemia/*metabolism
MH  - *Diterpenes
MH  - Enzyme Activation
MH  - Ginkgolides
MH  - Hippocampus/*metabolism
MH  - In Vitro Techniques
MH  - Isoquinolines/pharmacology
MH  - Lactones/*pharmacology
MH  - Phorbol 12,13-Dibutyrate/pharmacology
MH  - Piperazines/pharmacology
MH  - Platelet Activating Factor/*antagonists & inhibitors
MH  - Potassium/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tritium
EDAT- 1994/12/30 00:00
MHDA- 1994/12/30 00:01
CRDT- 1994/12/30 00:00
PHST- 1994/12/30 00:00 [pubmed]
PHST- 1994/12/30 00:01 [medline]
PHST- 1994/12/30 00:00 [entrez]
AID - 10.1097/00001756-199412300-00023 [doi]
PST - ppublish
SO  - Neuroreport. 1994 Dec 30;6(1):85-8. doi: 10.1097/00001756-199412300-00023.