PMID- 7704415
OWN - NLM
STAT- MEDLINE
DCOM- 19950509
LR  - 20191031
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 1
DP  - 1995 Jan
TI  - Detection of chromosome malsegregation to the daughter nuclei in 
      cytokinesis-blocked transgenic mouse splenocytes.
PG  - 45-53
AB  - Most of the recently developed tests for detecting aneugenic activity of 
      chemicals are based on the induction of micronuclei (MN) in cytokinesis-blocked 
      (CB) binucleated cells. In such a test, aneugens can be discriminated from 
      clastogens by checking for the presence of centromeres in the MN, indicating the 
      loss of whole chromosomes. Tracing particular chromosomes in interphase nuclei 
      using fluorescence in situ hybridization (FISH) with chromosome-specific DNA 
      probes is another method used for detecting numerical chromosome aberrations. 
      Here, we describe a method using a cytokinesis-blocked MN assay in combination 
      with identifying specific chromosomes of mice. For this purpose transgenic mice 
      with foreign DNA inserted in three pairs of their chromosomes were generated. 
      Splenocytes of these mice were cultured and treated in vitro with vinblastine 
      (VBL) or X-rays, followed by recovery in medium containing cytochalasin B. By 
      tracing the marker chromosomes in binucleated splenocytes, reciprocal products of 
      chromosome malsegregation to the daughter nuclei could be easily traced. The 
      results showed that besides clastogenic activity, X-rays also exhibited aneugenic 
      activity. Treatment with vinblastine showed a close relationship between 
      micronuclei induction and chromosome malsegregation, although at higher doses 
      malsegregation processes became more prominent. Simultaneous malsegregation of 
      more than one chromosome was observed frequently, but the three marker 
      chromosomes were found to be randomly involved in this process.
FAU - Boei, J J
AU  - Boei JJ
AD  - Department of Radiation Genetics and Chemical Mutagenesis, State University of 
      Leiden, The Netherlands.
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 5V9KLZ54CY (Vinblastine)
SB  - IM
MH  - Animals
MH  - *Cell Cycle
MH  - Cell Division
MH  - Cell Nucleus/*metabolism
MH  - *Chromosomes
MH  - Female
MH  - Mice
MH  - Mice, Transgenic
MH  - Spleen/*cytology/drug effects/radiation effects
MH  - Vinblastine/pharmacology
MH  - X-Rays
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF00711161 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Jan;3(1):45-53. doi: 10.1007/BF00711161.