PMID- 7705185
OWN - NLM
STAT- MEDLINE
DCOM- 19950511
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 19
IP  - 1
DP  - 1995 Jan 1
TI  - Semiautomated DNA probe mapping using digital imaging microscopy: I. System 
      development.
PG  - 51-9
AB  - Algorithms have been developed to help automate the mapping of DNA sequences 
      along metaphase chromosomes using fluorescence in situ hybridization (FISH). 
      Custom algorithms computationally define chromosome boundaries and compute 
      chromosomal medial axes. A dynamic regional thresholding (DRT) algorithm is 
      described that allows reliable detection of hybridization domains, even when they 
      differ substantially in size and intensity. Chromosomal locations are calculated 
      by determining the fractional location of each hybridization probe along the 
      medial axis of a metaphase chromosome relative to the short arm (FLpter). These 
      algorithms were tested on simulated data and by analysis of the location of 
      probes that had been previously mapped by other techniques. These algorithms 
      allow probes to be mapped rapidly along human chromosomes with a precision of 2-3 
      Mb.
FAU - Mascio, L N
AU  - Mascio LN
AD  - Engineering Research Division/Biology and Biotechnology Research Program, 
      Lawrence Livermore National Laboratory, Livermore, California, USA.
FAU - Verbeek, P W
AU  - Verbeek PW
FAU - Sudar, D
AU  - Sudar D
FAU - Kuo, W L
AU  - Kuo WL
FAU - Gray, J W
AU  - Gray JW
LA  - eng
GR  - HD 17665/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
SB  - IM
MH  - Algorithms
MH  - Chromosome Mapping/*methods
MH  - DNA Probes
MH  - Image Processing, Computer-Assisted/*methods
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Microscopy, Fluorescence/methods
MH  - *Software
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1002/cyto.990190107 [doi]
PST - ppublish
SO  - Cytometry. 1995 Jan 1;19(1):51-9. doi: 10.1002/cyto.990190107.